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The purpose of this study is to investigate Thiol-disulfide balance in patients with cervical preinvasive lesions
To date various oxidative stress paramaters were studied in patients with cervical preinvasive lesions, however dynamic thiol disulfide hemostasis has not been previously investigated. The thiol-disulfide balance in the circulation will be measured by the automated system created by Erel & Neselioglu(1).
References 1- Erel O, Neselioglu S. A novel and automated assay for thiol/ disulphide homeostasis. Clin Biochem 2014;47:326-32. doi:10.1016/ j.clinbiochem.2014.09.026.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| low grade squamous intraepithelial lesion(LGSIL) | low grade squamous intraepithelial lesion(LGSIL) n=100 |
| |
| high grade squamous intraepithelial lesion(HGSIL) | high grade squamous intraepithelial lesion(HGSIL) n=100 |
| |
| Healthy controls | Healthy volunteers n=100 |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Disulphide(μmol/L) | Diagnostic Test | Aforementioned oxidative stress markers will be measured via Cobas c501(Roche Diagnostics, Indianapolis, IN, USA) used with sphectrophotometry |
| Measure | Description | Time Frame |
|---|---|---|
| serum native thiol level | serum native thiol level in μmol/L | baseline (preoperative in the morning or preprandial for healthy volunteers) |
| serum disulphide level | serum disulphide level in μmol/L | baseline (preoperative in the morning or preprandial for healthy volunteers) |
| serum total thiol level | serum total thiol level in μmol/L | baseline (preoperative in the morning or preprandial for healthy volunteers) |
| the disulphide/native thiol ratio | the disulphide/native thiol ratio X (100 ) | baseline (preoperative in the morning or preprandial for healthy volunteers) |
| disulphide/total thiol ratio | disulphide/total thiol ratio X (100 ) | baseline (preoperative in the morning or preprandial for healthy volunteers) |
| native thiol/total thiol ratio | native thiol/total thiol ratio X (100 ) | baseline (preoperative in the morning or preprandial for healthy volunteers) |
| Measure | Description | Time Frame |
|---|---|---|
| total antioxidant capacity (TAC) | TAC in mmol trolox equivalent/L | baseline (preoperative in the morning or preprandial for healthy volunteers) |
| total oxidant capacity (TOC) | TOC in mmol H2O2 equivalent/L |
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Inclusion Criteria:
• Histopathologically confirmed diagnosis of LGSIL or HGSIL
Exclusion Criteria:
histopathologically confirmed LGSIL or HGSIL
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The diagnosis of preinvasive cervical lesions was made histopathologically by cervical biopsy. An experienced pathologist performed histopathologic examinations
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| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Mugla Sıtkı Kocman University Faculty of Medicine | Muğla | 48000 | Turkey (Türkiye) |
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| ID | Term |
|---|---|
| D000081483 | Squamous Intraepithelial Lesions |
| ID | Term |
|---|---|
| D065308 | Morphological and Microscopic Findings |
| D013568 | Pathological Conditions, Signs and Symptoms |
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|
| Total Oxidant Capacity(μmolH2O2Equiv/L) | Diagnostic Test | Aforementioned oxidative stress markers will be measured via Cobas c501(Roche Diagnostics, Indianapolis, IN, USA) used with sphectrophotometry |
|
|
| baseline (preoperative in the morning or preprandial for healthy volunteers) |
| Oxidative stress index (OSI) | OSI in mmol trolox/L. | baseline (preoperative in the morning or preprandial for healthy volunteers) |