Individuals with low copy numbers of salivary amylase gene have lower salivary amylase levels and might therefore have decreased capacity to metabolize starch into glucose. We will in a randomized crossover meal study examine the postprandial response of high-starch meals in individuals with either low or high copy number of the salivary amylase gene.

Inclusion Criteria:

• Participant in the Malmö Offspring Study
• Genotyped for AMY1 copy number variation and selected for having either low (2-4) or high (10 or above) copy number of AMY1
• Gender: both males and females
• Completed information on dietary habits

Exclusion Criteria:

• Allergic to wheat, gluten intolerant or on a low-carbohydrate diet
• Inability to eat up to 4 slices of white bread in 15 minutes
• Use of medication known to influence glucose metabolism, appetite or saliva flow
• Diagnosed diabetes or high fasting plasma glucose (above 6.1 mmol/L)
• Smoking

Genotype were blinded for the participants and all research staff except for the principal investigator. Amount of bread was not blinded.