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The aim of this study is to correlate and identify the biochemical markers which may reflect WOI. Blood samples will be collected and endometrial biopsy will be performed at particular time points.
Endometrial receptivity in the midluteal phase of the menstrual cycle, known as the window of implantation (WOI), occurs only for a very short period of time. However, so far, there is no reliable clinical and biochemical indicators of WOI. Therefore, this study is to correlate and identify the biochemical markers which may reflect WOI.
participants will be asked to be:
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| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| endometrial biopsy and blood sampling | Procedure | patient will be performed endometrial biopsy and collected blood samples at particular time points |
| Measure | Description | Time Frame |
|---|---|---|
| The change of the transcriptome profiling in the peripheral blood | RNA per sample will be prepared into two portions. One is for RNA sequencing and the other is for small RNA sequencing. The next step is hydrolysis of RNA into 200-300 nucleotides prior to reverse transcription. When sequencing RNA other than mRNA the library preparation is modified. The cellular RNA is selected based on the desired size range. For miRNA, the RNA is isolated through size selection. Once isolated, linkers are added to the 3' and 5' end then purified. The final step is cDNA generation through reverse transcription. | From the day of endometrial biopsy through study completion, an average of 2 months |
| The change of the immune markers in the peripheral blood | Immune markers including cytokines such as leukemia inhibitory factor, macrophage colony stimulating factor, interleukin - 1 beta, transforming growth factor beta-1, Tim-3, galectin-9 and other markers considered to play a role in implantation will also be measured. | From the day of endometrial biopsy through study completion, an average of 2 months |
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Inclusion Criteria:
Age 20-40 years.
Exclusion Criteria:
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This study only focuses on female group
Subjects will be recruited from the Prince of Wales Hospital.
| Name | Role | Phone | Extension | |
|---|---|---|---|---|
| Hiu Tan Margaret Lee | Contact | 85235051764 | margaretlee@cuhk.edu.hk | |
| Pui Wah Jacqueline Chung, MBBS | Contact | 85235051764 | jacquelinechung@cuhk.edu.hk |
| Name | Affiliation | Role |
|---|---|---|
| Pui Wah Jacqueline Chung, MBBS | Chinese University of Hong Kong | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Chinese University of Hong Kong | Recruiting | Hong Kong | Hong Kong |
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| ID | Term |
|---|---|
| D001800 | Blood Specimen Collection |
| ID | Term |
|---|---|
| D013048 | Specimen Handling |
| D019411 | Clinical Laboratory Techniques |
| D019937 | Diagnostic Techniques and Procedures |
| D003933 | Diagnosis |
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blood samples
| D011677 | Punctures |
| D013514 | Surgical Procedures, Operative |
| D008919 | Investigative Techniques |