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Randomized, blind parallel group design. Single intake of 55 g high Epicatechin /Melissa dark chocolate containing 160 mg Epicatechin per 55 g serving or single intake of 55 g low Epicatechin/ oat bran white chocolate containing < 0,00045 mg Epicatechin per 55 g serving.
A Total of 128 participants, 64 in each group, approx. equal number of male and female. There will be replacement of Drop-Outs until data from 128 participants are completed.
The primary endpoints will be performance in a pictorial memory task and a verbal memory task .
The secondary endpoints will be performance in a working memory test, Saliva cortisol, Visual analog scales assessing, anxiety, confidence, interference, solicitude, and excitement.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| High Epicatechin/ Melissa | Active Comparator | Single consumption of a 55g bar of dark chocolate containing: 42.8g Acticoa ® chocolate + 7.2g caster sugar + 5g Melissa containing 374 mg (-)-Epicatechin/100g chocolate and 2,69% of rosmarinic acid in Melissa leaves |
|
| Low Epicatechin/ Oat bran | Placebo Comparator | Single consumption of a 55g bar of white chocolate containing: 50g Lindor ® chocolate + 5g oat bran containing < 0,0009 mg (-)-Epicatechin/100g |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| High Epicatechin/ Melissa | Dietary Supplement | Single consumption of a 55g bar of dark chocolate containing: 42.8g Acticoa chocolate + 7.2g caster sugar + 5g Melissa |
|
| Measure | Description | Time Frame |
|---|---|---|
| Pictorial memory task | Participants will be presented with 24 neutral, 24 positive and 24 negative photographs in a random order. Scores will be calculated by summing the correctly remembered photographs per valence. | 25 minutes and 24 h after presentation |
| Verbal memory task | Six series of five semantically unrelated nouns will be presented.Total score is calculated by summing the number of correctly recalled words | 25 minutes and 24 h after presentation |
| Measure | Description | Time Frame |
|---|---|---|
| Saliva cortisol level | Samples are collected at different timepoints during the day in order to measure stress related cortisol activity. Participants have to chew a cotton sponge for 2-3 minutes, which then are contained in a special device | 1 day after word/picture presentation Start /middle and end of day. Baseline cortisol 1-14 days later. |
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Inclusion Criteria:
Exclusion Criteria:
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| Name | Affiliation | Role |
|---|---|---|
| Dominique DeQuervain, Prof. MD | University Basel Divison of Cognitive Neuroscience | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| University of Basel, Division of Cognitive Neuroscience | Basel | 4055 | Switzerland |
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| Low Epicatechin/ Oat bran | Other | Single consumption of a 55g bar of white chocolate containing: 50g Lindor chocolate + 5g oat bran |
|
| Working memory | Participants will have to calculate as many arithmetical means of seven one-digit numbers possible within two minutes by mental calculation. | 1 day after word/picture presentation |
| Visual Analogue Scale VAS test anxiety | will be assessed between each task by the participant using a visual analogue scale. Respondents specify their level of agreement to the statement by indicating a position along a continuous line (10 cm). | 1 day after word/picture presentation |
| Visual Analogue Scale VAS confidence | will be assessed between each task by the participant using a visual analogue scale. Respondents specify their level of agreement to the statement by indicating a position along a continuous line (10 cm). | 1 day after word/picture presentation |
| Visual Analogue Scale VAS interference | will be assessed between each task by the participant using a visual analogue scale. Respondents specify their level of agreement to the statement by indicating a position along a continuous line (10 cm). | 1 day after word/picture presentation |
| Visual Analogue Scale VAS solicitude | will be assessed between each task by the participant using a visual analogue scale. Respondents specify their level of agreement to the statement by indicating a position along a continuous line (10 cm). | 1 day after word/picture presentation |
| Visual Analogue Scale VAS excitement | will be assessed between each task by the participant using a visual analogue scale. Respondents specify their level of agreement to the statement by indicating a position along a continuous line (10 cm). | 1 day after word/picture presentation |