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| Name | Class |
|---|---|
| University of Birmingham | OTHER |
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10 young males will be recruited to participate in a randomized double blinded crossover study to investigate the influence of concomitant intake of two different types of carbohydrate with protein hydrolysate on the absorption kinetics of protein-derived amino acids and the impact on myofibrillar protein synthesis. During the trial days the subjects will perform a bout of whole body resistance exercise and ingest a supplement of meat hydrolysate labeled with D5-phenylalanine together with randomized and double blinded type of carbohydrate consisting of either glucose or cluster dextrin.
The primary outcome is the time to obtain peak concentration of D5-phenylalanine after intake of meat hydrolysate with either glucose or cluster dextrin.
Hypotheses:
I) The cluster dextrin will increase the absorption of the amino acids from the meat hydrolysate, and thereby result in a faster rise and a higher peak in plasma amino acid concentration in the postprandial period.
II) The meat hydrolysate will increase the protein synthesis rate to a higher extent when combined with cluster dextrin than with glucose.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Meat hydrolysate & Cluster Dextrin | Experimental | Meat hydrolysate containing 25g of protein given together with 75 grams of Cluster Dextrin. |
|
| Meat hydrolysate & placebo | Active Comparator | Meat hydrolysate containing 25g of protein given together with 75 grams of Glucose. |
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| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Meat Hydrolysate | Dietary Supplement | 25 grams of meat hydrolysate to maximally stimulate protein synthesis. |
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| Measure | Description | Time Frame |
|---|---|---|
| Protein hydrolysate amino acid label time to peak appearance in plasma | Difference between the time to peak of protein-derived D5-phenylalanine amino acid appearance in the plasma | From hour 0 to hour 3 |
| Measure | Description | Time Frame |
|---|---|---|
| Myofibrillar protein fractional synthesis rate response to protein and carbohydrate intake after a whole body resistance exercise | Difference between myofibrillar protein fractional synthesis rate after meat hydrolysate with either 75 grams of Cluster Dextrin or Clucose. | from hour 0,5 to hour hour 1. From hour 1 to hour 3. From hour 0,5 to hour 3. |
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Inclusion Criteria:
Exclusion Criteria:
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| Name | Affiliation | Role |
|---|---|---|
| Jacob L. Bülow, MD | Bispebjerg Hospital | Principal Investigator |
| Lars Holm, Prof. | University of Birmingham | Principal Investigator |
| Michael Kjaer, MD, prof. | Bispebjerg Hospital | Principal Investigator |
| Sofus Sølyst, stud.med. | University of Copenhagen | Principal Investigator |
| Mikkel Jensen, Stud.scient. | University of Copenhagen | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Bispebjerg Hospital | Copenhagen | Copenhagen NV | 2400 | Denmark |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 35182194 | Derived | Nishimura Y, Jensen M, Bulow J, Thomsen TT, Arimitsu T, van Hall G, Fujita S, Holm L. Co-ingestion of cluster dextrin carbohydrate does not increase exogenous protein-derived amino acid release or myofibrillar protein synthesis following a whole-body resistance exercise in moderately trained younger males: a double-blinded randomized controlled crossover trial. Eur J Nutr. 2022 Aug;61(5):2475-2491. doi: 10.1007/s00394-021-02782-y. Epub 2022 Feb 19. |
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| ID | Term |
|---|---|
| D036881 | Long-Term Synaptic Depression |
| D005947 | Glucose |
| ID | Term |
|---|---|
| D009473 | Neuronal Plasticity |
| D009424 | Nervous System Physiological Phenomena |
| D055687 | Musculoskeletal and Neural Physiological Phenomena |
| D006601 | Hexoses |
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| Cluster Dextrin | Dietary Supplement | 75 grams of Cluster Dextrin |
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| Placebo | Dietary Supplement | 75 grams of Glucose |
|
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| Protein hydrolysate amino acid label area under the concentration curve in plasma | Difference between the area under the concentration curve of protein-derived D5-phenylalanine amino acid appearance in the plasma | From hour 0 to hour 3 |
| Insulin concentration | Difference between insulin concentrations after intake of the two carbohydrate types. | From hour 0 to hour 3 |
| D009005 | Monosaccharides |
| D000073893 | Sugars |
| D002241 | Carbohydrates |