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Using stable isotope methodology, investigators will determine the postabsorptive and postprandial muscle protein synthetic response in 60 female adults (Age 50-79, BMI: <30 kg/m2) immediately after an acute bout of resistance exercise. Participants will be divided in to 5 groups of twelve women (n=12). Each group will receive a different protein beverage or placebo to consume following the resistance exercise training. Group 1 will consume a beverage consisting of whey protein; group 2 will consume whey protein supplemented with additional leucine; group 3 will consume whey protein supplemented with additional leucine and hydrolyzed whey peptides; group 4 will consume whey protein supplemented with additional leucine and citrulline; group 5 will just consume water. During the testing, blood and muscle samples will be collected.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Whey protein | Active Comparator | After resistance exercise, participants aged 50-79 years will consume 14g Whey protein dissolved in 200 mL of water |
|
| Whey + Leucine | Experimental | After resistance exercise, participants aged 50-79 years will consume 6.6 g Whey protein + 1.25 g Leucine dissolved in 200 mL of water |
|
| Whey + Leucine + Whey peptides | Experimental | After resistance exercise, participants aged 50-79 years will consume 4 g Whey protein + 1.25 g Leucine + 2.6 g Whey peptides dissolved in 200 mL of water |
|
| Whey + Leucine + Citrulline | Experimental | After resistance exercise, participants aged 50-79 years will consume 6.6 g Whey protein + 1.25 g Leucine + 0.8 g Citrulline dissolved in 200 mL of water |
|
| Water | Sham Comparator |
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Resistance Exercise | Behavioral | Participants will perform unilateral leg extension exercise immediately prior to ingestion of the experimental beverage |
|
| Measure | Description | Time Frame |
|---|---|---|
| The myofibrillar protein synthetic response to protein ingestion | Myofibrillar protein synthesis rates will be assessed during the postabsorptive period for 3 hr and during the 4 hr after the ingestion of the experimental beverages. This will allow us to assess the change from the postabsorptive to the postprandial period | Postabsorptive for 3 hours, Postprandial for 4 hours hours |
| Measure | Description | Time Frame |
|---|---|---|
| mTORC1 phosphorylation | Activation of anabolic signaling pathways will be assessed in the fasted state and at 2 and 4 hr after the ingestion of the experimental beverages. | Baseline and at 2 and 4 hours after protein ingestion |
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Inclusion Criteria:
Exclusion Criteria:
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| Name | Affiliation | Role |
|---|---|---|
| Nicholas A Burd, Ph.D. | University of Illinois at Chicago | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Freer Hall | Urbana | Illinois | 61801 | United States |
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After resistance exercise, participants aged either 20-30 or 50-79 years old will consume 200 mL water |
|
| 14 g Whey protein | Dietary Supplement |
|
| 6.6 g Whey protein + 1.25 g leucine | Dietary Supplement |
|
| 4 g Whey protein + 1.25 g leucine + 2.6 g Whey peptides | Dietary Supplement |
|
| 6.6 g Whey protein + 1.25 g leucine + 0.8 g Citrulline | Dietary Supplement |
|
| Water control | Other |
|
| ID | Term |
|---|---|
| D055070 | Resistance Training |
| D000067816 | Whey Proteins |
| D007930 | Leucine |
| D002956 | Citrulline |
| ID | Term |
|---|---|
| D005081 | Exercise Therapy |
| D012046 | Rehabilitation |
| D000359 | Aftercare |
| D003266 | Continuity of Patient Care |
| D005791 | Patient Care |
| D013812 | Therapeutics |
| D026741 | Physical Therapy Modalities |
| D064797 | Physical Conditioning, Human |
| D015444 | Exercise |
| D009043 | Motor Activity |
| D009068 | Movement |
| D009142 | Musculoskeletal Physiological Phenomena |
| D055687 | Musculoskeletal and Neural Physiological Phenomena |
| D008894 | Milk Proteins |
| D000080224 | Animal Proteins, Dietary |
| D004044 | Dietary Proteins |
| D011506 | Proteins |
| D000602 | Amino Acids, Peptides, and Proteins |
| D000067796 | Whey |
| D008892 | Milk |
| D003611 | Dairy Products |
| D005502 | Food |
| D000066888 | Diet, Food, and Nutrition |
| D010829 | Physiological Phenomena |
| D019602 | Food and Beverages |
| D000597 | Amino Acids, Branched-Chain |
| D000596 | Amino Acids |
| D000601 | Amino Acids, Essential |
| D000599 | Amino Acids, Diamino |
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