The purpose of the study is to evaluate the effect of various oral hygiene regimes on three volatile sulfur compounds.

Thirty male monk volunteers who matched with the including criteria were divided randomly into two groups. Both groups were practiced to brush their teeth with scrubbing method for one week. Then, each group were instructed to do different additional protocol regimen- group A have to do mouth washing and tooth brushing and group B have to practice tongue cleaning addition to tooth brushing for three weeks. At the fifth week, both groups were instructed to practice tooth brushing, mouth washing and tongue cleaning. Three different volatile sulfur compounds were measured by oral chroma. Tongue characteristics, oral examination were checked as the outcomes of this study.

Inclusion Criteria:

• Clinical diagnosis of oral malodor
• No systemic diseases and no severe dental diseases
• At least 20 functional teeth

Exclusion Criteria:

• Risky oral habit (smoking and betel quid chewing)
• Current use of antibiotics or any drugs
• Current use of any kind of mouthwash