Development and Validation of in Vitro Cell-based Bioassays for Nuclear Receptor Activation

Development and Validation of in Vitro Cell-based Bioassays for Nuclear Receptor Activation by Human Serum

This is a prospective biobank set up to collect serum from patients with disorders associated with changes in androgens, estrogens or sex hormone-binding globulin (SHBG), before and/or after treatment.

In vitro cell-based bioassays may be a technique to measure serum androgen or estrogen bioactivity. In this study investigators are collecting serum (and if possible, urine) samples from different cohorts of patients, before and/or after treatment.

The aim is to measure androgens, estrogens and SHBG as well as in vitro bioactivity with newly developed cell-based bioassays, and to compare different techniques to each other as well as to clinical endpoints available in the patient's Electronic Health Record.

Inclusion Criteria:

• Known or expected medical condition affecting androgens, estrogens or SHBG

Exclusion Criteria:

• Inability to provide written informed consent
• Known or suspected bloodborne infectious diseases (e.g. HIV, viral hepatitis etc.)
• Anemia (<11 g/dL hemoglobin) or being a Jehovah's Witness