Metabolic Availability of Tryptophan From White Maize

Application of the Indicator Amino Acid Oxidation Technique for the Determination of Metabolic Availability of Tryptophan From White Maize Protein, in Young Adult Men

Our objective is to determine the metabolic availability of Tryptophan in white maize using the indicator amino acid oxidation (IAAO) technique in adult men.

Our objective is to determine the metabolic availability of Tryptophan in white maize. Seven young, healthy, male adults will receive graded levels (13.2, 26.4, 39.7, 52.9) of tryptophan requirement of 3.78 mg/kg/d as a crystalline amino acid (AA) mixture and a porridge of white cornmeal protein respectively.

Inclusion Criteria:

• Healthy Adult males (20-49years old).
• Stable body weight
• Not on any medications that could affect protein and amino acid metabolism e.g. steroids.

Exclusion Criteria:

• Recent weight loss within the last 3 months or on weight reducing diet.
• Unwillingness to participate or unable to tolerate the diet.