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Our objective is to determine the metabolic availability of Lysine in white maize using the indicator amino acid oxidation (IAAO) technique in adult men.
Our objective is to determine the metabolic availability of Lysine in white maize. 7 young, healthy, male adults will receive graded levels (29, 37,46 and 54%) of lysine requirement of 35 mg/kg/d as a crystalline AA mixture and a porridge of white cornmeal protein respectively.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| levels of lysine intake. | Experimental | Randomly selected levels of lysine intake which are lower than the lysine requirement (previously derived). |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Crystalline Lysine Amino Acid Mix | Dietary Supplement | Crystalline Lysine from Amino Acid Mixture in amount of 29, 37, 46 and 54% of the mean Lysine requirement of 35 mg/kg/d will be given to subjects. |
| Measure | Description | Time Frame |
|---|---|---|
| Metabolic Availability of Lysine | 3 breath samples will be taken to provide a baseline F13CO2 measurement (Level of Phenylalanine oxidation). Breath samples will be collected and analyzed for 13CO2 enrichment. | Baseline |
| Measure | Description | Time Frame |
|---|---|---|
| Metabolic Availability of Lysine | 3 breath samples will be taken to provide a baseline F13CO2 measurement (Level of Phenylalanine oxidation). Breath samples will be collected and analyzed for 13CO2 enrichment. | 15 mins after intake of the fourth hourly meal (before tracer infusion) |
| Measure | Description | Time Frame |
|---|---|---|
| Metabolic availability of Lysine | 3 breath samples will be taken to provide a baseline F13CO2 measurement (Level of Phenylalanine oxidation). Breath samples will be collected and analyzed for 13CO2 enrichment. | 30 mins after intake of the fourth hourly meal (before tracer infusion) |
| Metabolic Availability of Lysine |
Inclusion Criteria:
Exclusion Criteria:
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| Name | Affiliation | Role |
|---|---|---|
| Paul B Pencharz, MD, PhD | The Hospital for Sick Children, Toronto, Canada | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| The Hospital for Sick Children | Toronto | Ontario | M5G-1X8 | Canada |
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| ID | Term |
|---|---|
| D002145 | Callosities |
| ID | Term |
|---|---|
| D007642 | Keratosis |
| D012871 | Skin Diseases |
| D017437 | Skin and Connective Tissue Diseases |
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| White corn meal porridge | Dietary Supplement | A cornmeal porridge including 29, 37, 46 and 54% of the mean Lysine requirement of 35 mg/kg/d will be given to subjects. |
|
3 breath samples will be taken to provide a baseline F13CO2 measurement (Level of Phenylalanine oxidation). Breath samples will be collected and analyzed for 13CO2 enrichment. |
| 45 mins after intake of the fourth hourly meal (before tracer infusion) |
| Metabolic Availability of Lysine | 4 breath samples will be taken to provide F13CO2 measurement (Level of Phenylalanine oxidation). Breath samples will be collected and analyzed for 13CO2 enrichment. | Post Tracer Infusion (between 150 and 240 min of tracer infusion) |