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| ID | Type | Description | Link |
|---|---|---|---|
| CIHR, grant MT10321 | Other Grant/Funding Number | CIHR, grant MT10321 |
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The purpose of this study is to investigate the digestibility of the amino acid lysine in cooked rice in adult men using stable isotope technique.
The investigators objective is to determine the metabolic availability (MA) of lysine in cooked white rice protein using the indicator amino acid oxidation (IAAO) technique. Five men will receive graded levels (20, 40, and 60, and 70%) of the lysine requirement of 35 mg/kg/d as a crystalline AA mixture,and cooked rice protein (20, 40 and 60%), respectively.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Lysine Amino Acid | Experimental |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Amino Acid Crystalline Lysine Amino Acid Mix | Dietary Supplement | crystalline lysine from AA MIX (20, 40, and 60% of the mean lysine requirement of 35 mg/kg/d) Lysine will be given in the form of L-Lysine-HCl, Sigma-Aldrich Brand. |
| Measure | Description | Time Frame |
|---|---|---|
| Metabolic Availability of lysine | 3 breath samples will be taken to provide a baseline F13CO2 measurement (Level of phenylalanine oxidation). Breath samples will be collected and analyzed for 13CO2 enrichment. | Baseline |
| Metabolic Availability of lysine | 3 breath samples will be taken to provide a baseline F13CO2 measurement (Level of phenylalanine oxidation). Breath samples will be collected and analyzed for 13CO2 enrichment. | 15 min after intake of the fourth hourly meal (before tracer infusion)) |
| Metabolic Availability of lysine | 3 breath samples will be taken to provide a baseline F13CO2 measurement (Level of phenylalanine oxidation). Breath samples will be collected and analyzed for 13CO2 enrichment. | 30 min after intake of the fourth hourly meal (before tracer infusion)) |
| Metabolic Availability of lysine | 3 breath samples will be taken to provide a baseline F13CO2 measurement (Level of phenylalanine oxidation). Breath samples will be collected and analyzed for 13CO2 enrichment. | 45 min after intake of the fourth hourly meal (before tracer infusion)) |
| Measure | Description | Time Frame |
|---|---|---|
| Metabolic Availability of Lysine | 4 breath samples will be taken to provide F13CO2 measurement (Level of phenylalanine oxidation).Breath samples will be collected and analyzed for 13CO2 enrichment. | Post Tracer Infusion (between 150 and 240 min of tracer infusion) |
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Inclusion Criteria:
Exclusion Criteria:
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| Name | Affiliation | Role |
|---|---|---|
| Paul B Pencharz, MD, PhD | The Hospital for Sick Children, Toronto, Canada | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| The Hospital for Sick Children | Toronto | Ontario | M5G-1X8 | Canada |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 23325920 | Derived | Prolla IR, Rafii M, Courtney-Martin G, Elango R, da Silva LP, Ball RO, Pencharz PB. Lysine from cooked white rice consumed by healthy young men is highly metabolically available when assessed using the indicator amino acid oxidation technique. J Nutr. 2013 Mar;143(3):302-6. doi: 10.3945/jn.112.166728. Epub 2013 Jan 16. |
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| Cooked Rice containing Lysine | Dietary Supplement | Lysine (20,40 and 60 % of the mean lysine requirement) from Cooked Rice |
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