In Vivo Quantification of Short Chain Fatty Acids Production in the Human Colon

In Vivo Quantification of Short Chain Fatty Acids Production in the Human Colon After Consumption of Different Cereal Fractions.

During this project the amount of SCFA produced in the colon after consumption of 2 different 13C-labelled wheat fiber fractions will be quantified.

Inclusion Criteria:

• Regular diet with 3 meals a day (minimal 5 times a week)
• BMI between 18.5 and 27 kg/m2

Exclusion Criteria:

• Calorie restricted diet or other special diet during the study or during the last month prior to the study
• Antibiotics in the month prior to the study
• Former surgeries on the gastrointestinal tract, with the exception of an appendectomy
• use of the following types of medications with effects on the gastrointestinal tract during the last 2 weeks prior to the study (spasmolytica, anti-diarrhea, anti-constipation, probiotics)
• Chronic disease of the gastrointestinal tract such as Crohn's disease, ulcerative colitis, irritable bowel syndrome, chronic constipation, chronic frequent diarrhea, clinically relevant lactose intolerance
• Pregnancy, pregnancy desire or breastfeeding
• Diabetes (type 1 and 2)
• Blood donation in the past 3 months prior to the study
• Abnormal hemoglobin (Hb) levels in blood, men must have a Hb level between 14.0 and 18.0 g / dL and women between 12.0 and 16.0 g / dL
• Participation in studies in the past year in which radiation was used.