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The aim of this study is to investigate wether change in endothelial nitric oxid synthase of blood cells (erythrocytes and peripheral blood mononuclear cells (PBMC)) has an influence on development and progression of cardiovascular diseases.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Healthy | 100 healthy volunteers in age of 20-75 years | ||
| Patients | 100 patients with stable coronary artery disease and accordingly endothelial dysfunction |
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| Measure | Description | Time Frame |
|---|---|---|
| Blood examination | Following parameters will be examined:
| baseline |
| Measure | Description | Time Frame |
|---|---|---|
| Protein expression | Determination with western blot | baseline |
| Asymmetric dimethylarginine (ADMA) and Cytokine | Determination with enzyme-linked immunosorbent assay (ELISA). |
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Inclusion Criteria:
Exclusion Criteria:
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100 healthy volunteers and 100 patient with stable coronary artery disease
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| Name | Affiliation | Role |
|---|---|---|
| Malte Kelm, Prof. MD | Department of Cardiology, Pneumology and Angiology, University Hospital Duesseldorf | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| University Hospital Duesseldorf | Düsseldorf | 40225 | Germany |
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| ID | Term |
|---|---|
| D002318 | Cardiovascular Diseases |
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Blood sample
| baseline |
| Microparticles | Determination with ELISA and flow cytometry | baseline |
| Immunocytochemistry | baseline |
| Nitric oxid metabolite and proteins in blood plasma, red blood cells and peripheral blood mononuclear cells (PBMN) | Determination with reductive chemiluminescence | baseline |
| Nitric oxid and reactive oxygen species | Determination with fluorescence coloring in flow cytometry and fluorescence microscopy | baseline |