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| ID | Type | Description | Link |
|---|---|---|---|
| 13-037 | Other Identifier | CTSU |
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The aim of this study is measurement of expression and activity of RBC eNOS in different clinical cohorts of patients with coronary artery disease and acute coronary syndrome and to investigate the importance of the NO-stimulated reaction cascade in terms of sGC and PKG.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Patients with CAD | Patients with stable coronary artery disease | ||
| Patients with ACS | Patients with acute coronar syndrome, instable Angina pectoris and low-risk NSTEMI | ||
| healthy control group | healthy control group without clinical apparent arteriosclerosis |
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| Measure | Description | Time Frame |
|---|---|---|
| Determination of red cell endothelial nitric oxide-synthase (eNOS)-expression and - activity | baseline, 3 hours and 3 month |
| Measure | Description | Time Frame |
|---|---|---|
| Deformability and stability of red blood cell membrane | by Laser assisted Optical Rotational Cell Analyzer (LORCA) | baseline, 3 hours and 3 month |
| Microvascular function | Determination of microvascular function with Laser-Doppler Perfusion Imaging |
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Inclusion Criteria:
Exclusion Criteria:
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3 groups:
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| Name | Affiliation | Role |
|---|---|---|
| Malte Kelm, Prof. MD | Department of Cardiology, Pneumology and Angiology, University Hospital Duesseldorf | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| University Hospital Duesseldorf | Düsseldorf | 40225 | Germany |
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blood samples
| baseline, 3 hours and 3 month |
| Peripheral and central hemodynamic measurement | baseline, 3 hours and 3 month |
| Determination of arterial stiffness | Determination of arterial stiffness with pulse wave analysis during blood pressure measurement or echocardiography. | baseline, 3 hours and 3 month |
| determination of flow-mediated dilation | baseline, 3 hours and 3 month |
| Determination of nitrate and nitrite in blood plasma and red blood cells (RBC) | baseline, 3 hours and 3 month |
| Determination of nitric oxide and cyclic guanosine monophosphate (cGMP) in blood plasma and in red blood cells | baseline, 3 hours and 3 month |
| Determination of endothelial microparticles | baseline, 3 hours and 3 month |
| Electrocardiogram | baseline, 3 hours and 3 month |
| Aging and turnover of red blood cells | baseline, 3 hours and 3 month |
| Redox status of red blood cells | Determination of redox status of red blood cells will be measured on free thiole with thioltracker and reactive oxygen species( ROS) by dihydrodichlorofluorescein | baseline, 3 hours and 3 month |
| Aggregation of red blood cells | Determination of aggregation of red blood cells with LORCA | baseline, 3 hours and 3 month |
| Platelet aggregometry | baseline, 3 hours and 3 month |
| Expression and function of soluble guanylyl cyclase (sGC) in red blood cells | measurement of enzyme expression and activity | baseline, 3 hours and 3 month |
| Expression and activity of Protein Kinase G (PKG) | measurement of enzyme expression and activity | baseline, 3 hours and 3 month |
| Determination of leucocyte populations and leucocyte function | flow-cytometry | baseline, 3 hours and 3 month |
| Changes of tetrahydrobiopterin-metabolism | baseline, 3 hours and 3 month |
| ID | Term |
|---|---|
| D003324 | Coronary Artery Disease |
| D054058 | Acute Coronary Syndrome |
| D000787 | Angina Pectoris |
| ID | Term |
|---|---|
| D003327 | Coronary Disease |
| D017202 | Myocardial Ischemia |
| D006331 | Heart Diseases |
| D002318 | Cardiovascular Diseases |
| D001161 | Arteriosclerosis |
| D001157 | Arterial Occlusive Diseases |
| D014652 | Vascular Diseases |
| D002637 | Chest Pain |
| D010146 | Pain |
| D009461 | Neurologic Manifestations |
| D012816 | Signs and Symptoms |
| D013568 | Pathological Conditions, Signs and Symptoms |
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