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The purpose of the study is to investigate the metabolic effects during acute inflammation with and without the nutritional supplement of amino acids.
E. Coli Endotoxin (LPS, lipopolysaccharide) is used to initiate an inflammatory response.
The study is an interventional randomized placebo study including 8 healthy male subjects. Each subject participates 3 times (different days) and are given one of following interventions:
It is our hypothesis that the nutritional intervention during acute inflammation plays an important role in lipid and protein metabolism.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Saline | No Intervention | Only saline is given. | |
| + Saline | Placebo Comparator | Endotoxin is given + Placebo (Saline) |
|
| + amino acids | Active Comparator | Endotoxin is given + amino acids (intravenously) |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| E. coli endotoxin, US standard | Biological |
|
| Measure | Description | Time Frame |
|---|---|---|
| Protein catabolism during acute illness | Protein catabolism is measured with amino acid tracer techniques comparing radioactive marked amino acids from blood samples. | After 6 hours intervention |
| Measure | Description | Time Frame |
|---|---|---|
| The local effects on muscle and fat tissue | A muscle + fat biopsy are taken twice during the intervention to focus on the local effects in these tissues. | At 2 hours and 4,5 hours |
| Other systemic metabolic effects during acute illness |
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Inclusion Criteria:
Exclusion Criteria:
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| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Medical Department MEA, NBG, Aarhus University Hospital | Aarhus C | Jylland | 8000 | Denmark |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 37844838 | Derived | Pedersen MGB, Lauritzen ES, Svart MV, Stoy J, Sondergaard E, Thomsen HH, Kampmann U, Bjerre M, Jessen N, Moller N, Rittig N. Nutrient sensing: LEAP2 concentration in response to fasting, glucose, lactate, and beta-hydroxybutyrate in healthy young males. Am J Clin Nutr. 2023 Dec;118(6):1091-1098. doi: 10.1016/j.ajcnut.2023.10.007. Epub 2023 Oct 14. | |
| 35883493 |
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| ID | Term |
|---|---|
| D007249 | Inflammation |
| D008659 | Metabolic Diseases |
| ID | Term |
|---|---|
| D010335 | Pathologic Processes |
| D013568 | Pathological Conditions, Signs and Symptoms |
| D009750 | Nutritional and Metabolic Diseases |
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Lipid and glucose metabolism are measured with tracer techniques with radioactive marked glucose + lipid.
| 6 hours |
| Vladimirova YV, Molmer MK, Antonsen KW, Moller N, Rittig N, Nielsen MC, Moller HJ. A New Serum Macrophage Checkpoint Biomarker for Innate Immunotherapy: Soluble Signal-Regulatory Protein Alpha (sSIRPalpha). Biomolecules. 2022 Jul 4;12(7):937. doi: 10.3390/biom12070937. |
| 27627109 | Derived | Rittig N, Bach E, Thomsen HH, Pedersen SB, Nielsen TS, Jorgensen JO, Jessen N, Moller N. Regulation of Lipolysis and Adipose Tissue Signaling during Acute Endotoxin-Induced Inflammation: A Human Randomized Crossover Trial. PLoS One. 2016 Sep 14;11(9):e0162167. doi: 10.1371/journal.pone.0162167. eCollection 2016. |
| 25896101 | Derived | Rittig N, Bach E, Thomsen HH, Johannsen M, Jorgensen JO, Richelsen B, Jessen N, Moller N. Amino acid supplementation is anabolic during the acute phase of endotoxin-induced inflammation: A human randomized crossover trial. Clin Nutr. 2016 Apr;35(2):322-330. doi: 10.1016/j.clnu.2015.03.021. Epub 2015 Apr 9. |