Effects of Free Fatty Acids and 3-hydroxybutyrate on Protein, Glucose, Lipid Metabolism and Intracellular Signals.

Effects of Free Fatty Acids and 3-hydroxybutyrate on Protein, Glucose, Lipid Metabolism and Intracellular Signals; a Study on Muscle Wasting and Metabolism During Acute Inflammation and Potential Anabolic Mechanisms.

The investigators hypothesize that the investigators can detect protein-sparing effects of administration of the ketone 3-hydroxybutyrate and free fatty acids during simulation of an acute inflammatory disease. The investigators use the infusion of endotoxin, US standard reference E.coli in healthy subjects as a model for inflammation / infection and to evaluate the effect on protein metabolism using different tracers and the investigators can measure the various intracellular signaling pathways of selected muscle and adipose tissue.

Inclusion Criteria:

• 20 < body mass index < 30 kg/m2.
• written and orally informed consent before enrollment.

Exclusion Criteria:

• participation in other studies using radioactive tracers or other exposure to radiation (X-rays, scintigraphy etc).
• allergy to soy products or eggs
• diabetes, any type
• epilepsy
• ongoing infection
• immune deficiency
• cardiovascular disease
• dysregulated hypertension
• primary muscles disease, congenital or acquired