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| ID | Type | Description | Link |
|---|---|---|---|
| BB/1005862/1 | Other Grant/Funding Number | BBSRC |
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| Name | Class |
|---|---|
| Royal Veterinary College | UNKNOWN |
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Many types of cardiovascular disease begin when the layer of cells lining blood vessels (endothelial cells) start to function abnormally. This causes white blood cells (monocytes) to enter the blood vessel wall and eventually form lesions. Fats from foods we consume are carried in the blood for 3-8 hours after a fatty meal in small particles known as chylomicrons (CM) and chylomicron remnants (CMR). The overall aim of this project is to investigate the idea that n-3 polyunsaturated fatty acids (PUFA) protect against heart disease by modifying the effect of CMR on endothelial cells and monocytes. We hypothesize that n3-PUFA carried in CMR reduce detrimental events which promote blood vessel damage and activate protective mechanisms to improve the function of arteries.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Oleic acid | Active Comparator | 75 g high oleic acid sunflower oil. |
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| Linoleic acid | Active Comparator | 75 g high linoleic acid sunflower oil. |
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| Eicosapentaenoic acid and docosahexaenoic acid | Experimental | 5 g EPA and DHA derived from fish oil, made up to a total of 75 g with high oleic sunflower oil. |
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| Docosahexaenoic acid | Experimental | 5 g DHA derived from algal oil, made up to a total of 75 g with high oleic sunflower oil. |
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| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| High-fat meals varying in their fatty acid composition | Dietary Supplement | 70 g fat incorporated into a muffin and milkshake meal, consumed following fasting baseline measurements |
| Measure | Description | Time Frame |
|---|---|---|
| Activation of inflammatory/oxidative stress pathways within cultured endothelial cells following treatment with 6 h postprandial chylomicron remnant-rich lipoprotein fraction | The primary outcome of the study is activation of inflammatory/oxidative stress pathways within cultured endothelial cells following incubation with pooled postprandial lipoprotein fractions rich in chylomicron remnants. Due to the nature of this type of research this necessitates more than one primary outcome measure: the primary measures are NF-kappa-beta activation, cytokine production (e.g. interleukin-6) and reactive oxygen species generation in the cultured human endothelial cells. | 6 h post-meal |
| Measure | Description | Time Frame |
|---|---|---|
| Incremental area under the plasma concentration versus time curve (iAUC) of triacylglycerol | 0, 1, 2, 3, 4, 5 and 6 h post-meal | |
| Incremental area under the plasma concentration versus time curve (iAUC) of glucose | 0, 1, 2, 3, 4, 5 and 6 h post-meal |
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| Name | Affiliation | Role |
|---|---|---|
| Wendy L Hall, PhD | King's College London | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Diabetes & Nutritional Sciences Division, King's College London | London | SE1 9NH | United Kingdom |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 8881715 | Background | Lambert MS, Botham KM, Mayes PA. Modification of the fatty acid composition of dietary oils and fats on incorporation into chylomicrons and chylomicron remnants. Br J Nutr. 1996 Sep;76(3):435-45. doi: 10.1079/bjn19960048. | |
| 16305504 | Background | Botham KM, Bravo E, Elliott J, Wheeler-Jones CP. Direct interaction of dietary lipids carried in chylomicron remnants with cells of the artery wall: implications for atherosclerosis development. Curr Pharm Des. 2005;11(28):3681-95. doi: 10.2174/138161205774580732. |
| Label | URL |
|---|---|
| Related Info | View source |
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| ID | Term |
|---|---|
| D006949 | Hyperlipidemias |
| D000783 | Aneurysm |
| ID | Term |
|---|---|
| D050171 | Dyslipidemias |
| D052439 | Lipid Metabolism Disorders |
| D008659 | Metabolic Diseases |
| D009750 | Nutritional and Metabolic Diseases |
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| Incremental area under the plasma concentration versus time curve (iAUC) for non-esterified fatty acids | 0, 1, 2, 3, 4, 5 and 6 h post-meal |
| Incremental area under the plasma concentration versus time curve (iAUC) for plasma fatty acid composition (%) | 0, 1, 2, 3, 4, 5 and 6 h post-meal |
| Incremental area under the plasma concentration versus time curve (iAUC) for cholesterol | 0, 1, 2, 3, 4, 5 and 6 h post-meal |
| Incremental area under the unit measure versus time curve for brachial augmentation index | 0, 30, 60, 90, 120, 150, 180, 210, 240, 270, 300, 330 and 360 min post-meal |
| Incremental area under the unit measure versus time curve for systolic blood pressure | 0, 30, 60, 90, 120, 150, 180, 210, 240, 270, 300, 330 and 360 min post-meal |
| Incremental area under the unit measure versus time curve for diastolic blood pressure | 0, 30, 60, 90, 120, 150, 180, 210, 240, 270, 300, 330 and 360 min post-meal |
| Change in digital volume pulse stiffness index | 0, 2, 4 and 6 h post-meal |
| Change in digital volume pulse reflection index | 0, 2, 4 and 6 h post-meal |
| Change in plasma nitrite/nitrate concentrations | 0, 2, 4 and 6 h |
| Change in plasma 8-isoprostane F2alpha concentrations | 0, 2, 4 and 6 h post-meal |
| Activation of inflammatory/oxidative stress pathways within cultured endothelial cells following treatment with 4 h postprandial chylomicron remnant-rich lipoprotein fraction | 4 h post-meal |
| Activation of inflammatory/oxidative stress pathways within cultured endothelial cells following treatment with 5 h postprandial chylomicron remnant-rich lipoprotein fraction | 5 h post-meal |
| 12352009 | Background | Proctor SD, Vine DF, Mamo JC. Arterial retention of apolipoprotein B(48)- and B(100)-containing lipoproteins in atherogenesis. Curr Opin Lipidol. 2002 Oct;13(5):461-70. doi: 10.1097/00041433-200210000-00001. |
| 10974050 | Background | Marcoux C, Hopkins PN, Wang T, Leary ET, Nakajima K, Davignon J, Cohn JS. Remnant-like particle cholesterol and triglyceride levels of hypertriglyceridemic patients in the fed and fasted state. J Lipid Res. 2000 Sep;41(9):1428-36. |
| 18203893 | Background | Hall WL, Sanders KA, Sanders TA, Chowienczyk PJ. A high-fat meal enriched with eicosapentaenoic acid reduces postprandial arterial stiffness measured by digital volume pulse analysis in healthy men. J Nutr. 2008 Feb;138(2):287-91. doi: 10.1093/jn/138.2.287. |
| 19138437 | Background | Burdge GC, Powell J, Dadd T, Talbot D, Civil J, Calder PC. Acute consumption of fish oil improves postprandial VLDL profiles in healthy men aged 50-65 years. Br J Nutr. 2009 Jul;102(1):160-5. doi: 10.1017/S0007114508143550. Epub 2009 Jan 13. |
| 7889892 | Background | Zampelas A, Peel AS, Gould BJ, Wright J, Williams CM. Polyunsaturated fatty acids of the n-6 and n-3 series: effects on postprandial lipid and apolipoprotein levels in healthy men. Eur J Clin Nutr. 1994 Dec;48(12):842-8. |
| 18052925 | Background | Armah CK, Jackson KG, Doman I, James L, Cheghani F, Minihane AM. Fish oil fatty acids improve postprandial vascular reactivity in healthy men. Clin Sci (Lond). 2008 Jun;114(11):679-86. doi: 10.1042/CS20070277. |
| 22348439 | Background | Rontoyanni VG, Hall WL, Pombo-Rodrigues S, Appleton A, Chung R, Sanders TA. A comparison of the changes in cardiac output and systemic vascular resistance during exercise following high-fat meals containing DHA or EPA. Br J Nutr. 2012 Aug;108(3):492-9. doi: 10.1017/S0007114511005721. Epub 2012 Feb 21. |
| 25099540 | Derived | Purcell R, Latham SH, Botham KM, Hall WL, Wheeler-Jones CP. High-fat meals rich in EPA plus DHA compared with DHA only have differential effects on postprandial lipemia and plasma 8-isoprostane F2alpha concentrations relative to a control high-oleic acid meal: a randomized controlled trial. Am J Clin Nutr. 2014 Oct;100(4):1019-28. doi: 10.3945/ajcn.114.091223. Epub 2014 Aug 6. |
| D014652 | Vascular Diseases |
| D002318 | Cardiovascular Diseases |