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| Name | Class |
|---|---|
| Unity Health Toronto | OTHER |
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Eight healthy adult subjects will be given a meal of one, two or four cups red raspberries, or two slices white bread with or without 200 mg vitamin C, after an overnight fast and consumption of a low polyphenol diet for two days. Blood samples will be taken at various time points over an eight hour period to measure oxidative stress and antioxidant levels in the blood. Each subject will attend 5 study visits with one week intervals and be given each meal in a random order. It is hypothesized that raspberry consumption at higher doses will greater protect against meal-induced oxidative stress compared to bread controls.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| one cup red raspberries | Experimental | treatment meal consists of one cup red raspberries |
|
| two cups red raspberries | Experimental | treatment meal consists of two cups red raspberries |
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| four cups red raspberries | Experimental | treatment meal consists of four cups red raspberries |
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| bread | Placebo Comparator | treatment meal consists of two slices white bread |
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| vitamin C | Active Comparator | treatment meal consists of two slices white bread and 200 mg vitamin C in the form of supplemental ascorbic acid |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| red raspberries | Dietary Supplement | single serving of one, two or four cups red raspberries |
|
| Measure | Description | Time Frame |
|---|---|---|
| change in antioxidant capacity from baseline (-0.25 h) | plasma antioxidant capacity measured using the 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) radical cation assay and expressed in Trolox Equivalents (TE) | -0.25, 0.25, 0.5, 1, 2, 4 hours post-intake |
| Measure | Description | Time Frame |
|---|---|---|
| change in lipid peroxidation from baseline (-0.25 h) | serum thiobarbituric acid reactive substances (TBARS) measured as an indicator of the lipid peroxidation product malondialdehyde (MDA) | -0.25, 0.25, 0.5, 1, 2, 4 hours post-intake |
| change in thiols compared to baseline (-0.25 h) |
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Inclusion Criteria:
Exclusion Criteria:
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| Name | Affiliation | Role |
|---|---|---|
| A V Rao, PhD | University of Toronto | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Clinical Nutrition and Risk Factor Modification Centre; St. Michael's Hospital | Toronto | Ontario | M5C 2T2 | Canada |
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| bread controls | Dietary Supplement | two slices white bread alone or with 200 mg vitamin C supplement |
|
plasma thiols quantified by 5,5'-dithiobis-(2-nitrobenzoic acid) (DTNB) assay as an indicator of the level of protection against protein oxidation |
| -025, 0.25, 0.5, 1, 2, 4 hours post-intake |