Biomarkers in Tissue and Blood Samples From Patients With Early-Stage Non-Small Cell Lung Cancer

Epigenetic Changes as Prognostic Markers in Patients With Early Stage Non-Small Cell Lung Cancer

RATIONALE: Studying samples of blood and tumor tissue from patients with cancer in the laboratory may help doctors learn more about changes that occur in DNA and identify biomarkers related to cancer.

PURPOSE: This research study is studying biomarkers in tissue and blood samples from patients with early-stage non-small cell lung cancer.

OBJECTIVES:

• Evaluate aberrant methylation patterns in tissue and serum samples from patients with early-stage non-small cell lung cancer (NSCLC) to validate the Johns Hopkins single institutional study.
• Attempt to define subgroups of patients at greater risk for recurrent or metastatic disease who may benefit from more aggressive adjuvant therapeutic regimens.
• Develop prognostic indicators for disease-specific and overall survival.
• Define new potential molecular targets for therapy.

OUTLINE: Archived tumor and intrathoracic lymph node tissue samples are analyzed for aberrant DNA methylation (p16/CDKN2A, DAP kinase, H-cadherin, APC, and RASSF1A) by methylation-specific PCR. Analyses are then compared with the preliminary data from the Johns Hopkins institutional study.

DISEASE CHARACTERISTICS:

• Diagnosis of early-stage non-small cell lung cancer

  • T1 or T2, N0 disease
  • Had a lobectomy or greater resection with negative surgical margins (R0 resection)

• Available tumor and lymph node specimens from patients enrolled on ACOSOG-Z0040 and ACOSOG-Z0030 trials

  • Node-negative with recurrent disease within 3 years (cases)
  • Node-negative patients without recurrent disease (controls)

PRIOR CONCURRENT THERAPY:

• See Disease Characteristics