Effects of an Amino-acid Supplement on Hepatic Lipid Metabolism

Effects of a Supplementation With Amino-acids on Hepatic Lipid Metabolism

10 healthy male volunteers will be studied after

• a 6 day weight maintenance, balanced diet
• a 6 days weight maintenance, balanced diet supplemented with 3 grams fructose/kg body weight/day
• a 6 days weight maintenance, balanced diet supplemented with 3 grams fructose/kg body weight/day and 20g amino-acids per day At the end of each 6 days period, the following measurements will be obtained
• intrahepatic lipids (1H-MRS)
• metabolic effects of fructose ingestion (measurement of substrate oxidation, gluconeogenesis from fructose, palmitate synthesis from fructose, plasma VLDL-kinetics)

This is a randomized, double blinded study

Inclusion Criteria:

• age 18-30 years
• sex: males
• BMI between 19 and 25 kg/m2
• less than 3 30min- exercise session/week

Exclusion Criteria:

• smokers
• alcohol consumption more than 50g/week
• consumption of drugs
• history of diabetes in first degree relatives
• presence of ferro-magnetic prosthesis, cardiac pacemakers, or any contra-indication to NMR