The primary aims of this study are to assess the effects of habitual dietary protein intakes across the acceptable macronutrient distribution range with lean beef/pork or soy/legumes as the predominate sources of protein on indices of daily appetite and mood, and on postprandial appetite, mood, energy expenditure, and glycemic responses during energy-restricted weight loss in overweight adults.

Inclusion Criteria:

• Age range: 21 years and older
• Body mass index between 27.0-36.9 kg/m2
• Non-smoking (within the last 6 months)
• Weight stable (< 3 kg (~7 lbs) weight gain or loss within last 3 months)
• Energy need for weight maintenance 1950-2750 kcal/day
• Not dietary restrained
• Menstruating women not pregnant or lactating
• Constant habitual activity patterns (within last 3 months)
• No Acute Illness (or have chronic diseases known to influence protein or energy metabolism)
• Non-diabetic
• Clinically normal blood profiles (within 10% of clinical normalcy)
• Non-hypertensive
• Not taking medications known to influence appetite or metabolism
• Willingness to eat study foods
• Able to travel to testing facility

Exclusion Criteria:

• Age: <21 years
• Body mass index: outside of the 27.0-36.9 kg/m2 range
• Smoker (currently or within the last 6 months)
• Gained or lost > 3.0 kg (7 lbs) within the last 3 months
• Energy need for weight maintenance < 1950 or > 2750 kcal/day
• Dietary restrained (≥ 14 on Three Factor Eating Questionnaire)
• Pregnant, lactating, or non-menstruating women
• Clinically diagnosed as a diabetic, or with liver or kidney disease/dysfunction, or osteoporosis
• Clinically abnormal blood profiles as identified by our study physician, Arthur Rosen, MD
• Hypertensive
• Taking medications (currently or within the last 3 months) known to influence appetite or metabolism
• Allergies to eggs
• Lactose intolerance
• Unwillingness to eat study foods
• Inability to travel to testing facility