Embryo Development in Microfluidics System of Culture

Comparison of the in Vitro Development of Human Embryos in Static and and Microfluidics Systems of Culture

Embryo culture is a cornerstone of in vitro fertilization treatments. Usually, embryos are cultured in microdrops of culture media in incubators with controlled temperature, humidity and atmosphere. However, these culture systems are static, while in vivo the initial growth of the embryo takes place in the uterine tube and they are submitted to a dynamic environment. The aim of this study is to compare the embryo development on days 2 and 3 of culture morphological features of embryos under static and dynamic(microfluidics) systems of culture.

Embryo culture is a cornerstone of in vitro fertilization treatments. Usually, embryos are cultured in microdrops of culture media in incubators with controlled temperature, humidity and atmosphere. However, these culture systems are static, while in vivo the initial growth of the embryo takes place in the uterine tube and they are submitted to a dynamic environment. The aim of this study is to compare the embryo development on days 2 and 3 of culture morphological features of embryos under static and dynamic(microfluidics) systems of culture.

Inclusion Criteria:

• Age between 21 and 35 years old
• No more than 1 previous IVF failure
• Absence of physical conditions that can interfere in oocyte quality
• At least 8 zigotes 24 hours after fertilization

Exclusion Criteria:

• Use of epididimary or testicular sperm
• Sperm or egg donation
• Untreated systemic diseases