Gut Hormone Secretion and Macronutrients

Gut Hormone Secretion Pattern in Response to Different Macronutrients in Healthy Volunteers

The role of gut hormone (GLP-1, GIP, PYY...) in glucose homeostasis has been widely demonstrated. Furthermore modifications in their pattern of secretion seem to be involved in the improvement of glucose control in obese patients after bariatric surgery.

The purpose of this study is to assess the respective role of carbohydrate, fat and protein from a complete meal in gut hormones secretion.

The study is designed to asses the role of each individual macronutrient of a compete meal in gut hormones secretion in healthy volunteers.

8 healthy young male subjects will be studied on 5 different occasions. To asses the role of each macronutient the subjects will recieve one of the following test meal: a complete sandwich, the proteic part of this complete meal, the glucidic part and the lipidic part. The fifth test will be a fasting test.

To assess the entero-insular axis, a 5.5 hours hyperglycaemic clamp will be performed.

Inclusion Criteria:

• Male
• 18-35 years old
• 19 kg/m2 ≤ BMI ≤ 25 kg/m2
• Healthy
• Low to moderate physical activity
• Non smokers

Exclusion Criteria:

• Family history of diabetes mellitus
• Any medication
• Participation to blood spending or other clinical study in the 3 months before the beginning of this study
• Consumption of drugs
• Consumption of more than 50g of alcool in a week
• Vegetarian or particular diet
• Anamnèse d'intolérance alimentaire
• Family history of food intolerance