Effects of Fasting and Exercise on Insulin and GH Signaltransduction in Muscle and Fat

Effects of Fasting and Exercise on Insulin and GH Signaltransduction in Muscle and Fat

Hypothesis: under fasting there will primarily be an activation of growth hormone(GH) signalpathways and a inhibition of insulin signalpathways, and the opposite will happen under physical activity.

Primarily we will investigate markers for GH and insulin signaltransduction in muscle- and fat-biopsies after fasting for 72 hours and after 1 hour of ergometer-cycling at 65% Vo2max.

In addition to this we will investigate the effect of fasting and exercise on the velocity of carbamide synthesis, aminoacid rate of turnover and glucose rate of turnover.

Inclusion Criteria:

• healthy men
• Body mass index (BMI) 20-26
• age 18-45 years

Exclusion Criteria:

• any kind of disease
• any use of medicine