The Influence of Different Concentrations of Glucose in Culture Media on Fertilization and Embryo Quality

The Influence of Different Concentrations of Glucose in Culture Media(i.e: ISM1 vs. IVF) on Fertilization and Embryo Quality

The aim of this study is to examine whether incubation of human oocytes until pronuclear stage, in different culture media containing high and low concentrations of glucose affect fertilization differently, not only in standard IVF cycles, but also after intracytoplasmatic sperm injection.In addition, we wish to determine whether exposure of oocytes to different concentrations of glucose until pronuclear stage has further effect on embryo quality and development until the blastocyst stage.

Inclusion Criteria:

• Patients undergoing IVF treatment in our clinic during study period

Exclusion Criteria:

• Females older than 40 years
• Patients with less than 6 retrieved oocytes
• Male patients older than 50 years old
• Patients after testis biopsies