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NCT07736716

Overview

NCT ID: NCT07736716
Lead Sponsor: Ankara City Hospital BilkentOTHER
Official Title: Arthroscopic Mechanical Release of the Infrapatellar Fat Pad Mobilizes Cells With a Mesenchymal Stem Cell Immunophenotype and Alters Early Molecular Responses.
Brief Summary: This prospective, single-center, paired-sample clinical laboratory study evaluated whether controlled mechanical release of the infrapatellar fat pad during knee arthroscopy increases the number of intra-articular cells with a mesenchymal stem cell immunophenotype. The study also evaluated whether the procedure is accompanied by immediate changes in the expression of selected genes associated with inflammation, immunomodulation, and tissue repair.

Adults undergoing knee arthroscopy for meniscal pathology had a 20-mL intra-articular fluid sample collected before manipulation of any intra-articular structure. The infrapatellar fat pad was then gently and selectively shaved for approximately 1 minute using a motorized arthroscopic shaver with the suction turned off. A second 20-mL intra-articular fluid sample was collected immediately after the procedure and before further irrigation or continuation of the planned arthroscopic surgery.

Paired pre-release and post-release samples were analyzed by flowcytometry to quantify cells with a mesenchymal stem cell immunophenotype. Quantitative real-time polymerase chain reaction was performed on the total cellular fraction to assess IL-1β, IL-6, IL-10, TGF-β, and CD24 gene expression. Comparisons were performed within each participant using the paired samples.

Status and Timeline

Status: Completed
Last Update Posted: Jul 30, 2026Actual
Start Date: Jan 9, 2024Actual
Primary Completion Date: Jan 10, 2025Actual
Completion Date

Participation Criteria

Ages Eligible for Study: Adult (18-64)
Sexes Eligible for Study: All
Accepts Healthy Volunteers?: No
Eligibility Criteria: Inclusion Criteria:

Age between 18 and 55 years

Presence of knee pain

Meniscal tear confirmed by magnetic resonance imaging

Scheduled to undergo arthroscopic knee surgery

Provision of written informed consent to participate in the study

Exclusion Criteria:

Requirement for emergency surgery

Open injury involving the knee

Severe anterior or posterior cruciate ligament injury causing substantial knee instability

Tibial eminence fracture

Osteochondral defect requiring surgical treatment

Planned open knee surgery

Suspected septic arthritis

Inflammatory arthritis

Suspected intra-articular tumor

Previous arthroscopic surgery on the same knee

Declining to participate in the study

Study Plan

Study Type: Interventional
Phase: Not Applicable
Enrollment: 26Actual
Primary Purpose: Basic Science
Allocation: N/A
Interventional Model: Single Group Assignment
Masking: None (Open Label)

Contacts and Locations

Central Contacts: No data available
Locations: Ankara Bilkent City Hospital

Similar Studies

Participant Group/Arms

Arthroscopic Infrapatellar Fat Pad ReleaseExperimental

Participants undergoing routine knee arthroscopy for meniscal pathology underwent paired intra-articular fluid sampling. A 20-mL baseline sample was collected before any intra-articular manipulation. The infrapatellar fat pad was then gently and selectively shaved for approximately 1 minute using a motorized arthroscopic shaver with the suction temporarily turned off. A second 20-mL intra-articular fluid sample was collected immediately after shaving and before further irrigation. The released tissue was subsequently removed using routine arthroscopic suction and irrigation, and the planned arthroscopic procedure was completed.

Procedure: Controlled Mechanical Release of the Infrapatellar Fat Pad