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| Name | Class |
|---|---|
| EMO Biomedicine Corporation | INDUSTRY |
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According to many recent research, mesenchymal stem cells (MSC) isolated from synovial membrane and infrapatellar fat pad have higher ex vivo proliferation capacity, multipotency and ability to undergo chondrogenesis.
Based on this concept, the investigators plan to get two removed tissues (synovial membrane and infrapatellar fat pad) from patients who have an operation of total knee replacement. The investigators are going to isolate and expand MSC from these two tissues respectively, and then compare the chondrogenesis ability for clinical application. In the other hand, the investigators plan to build techniques and procedures which comply with "Good Tissue Practices (GTP)"
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| 103024-F | For patients who have an operation of total knee replacement, two removed tissues (synovial membrane and infrapatellar fat pad) will be collected for mesenchymal stem cell isolation. |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| 103024-F | Other | mesenchymal stem cell isolation |
|
| Measure | Description | Time Frame |
|---|---|---|
| Cell viability ratio of synovial membrane MSC and infrapatellar fat pad MSC from the first to the tenth cell passage | 6 months | |
| Doubling time of synovial membrane MSC and infrapatellar fat pad MSC from the first to the tenth cell passage | 6 months | |
| Percentage expression of cluster differentiation 90,105, 34, 45 surface markers of synovial membrane MSC and infrapatellar fat pad MSC from the first to the tenth cell passage | 6 months |
| Measure | Description | Time Frame |
|---|---|---|
| Alcian blue staining of synovial membrane MSC and infrapatellar fat pad MSC | 6 months | |
| Tyep II collagen and aggrecan gene expression of synovial membrane MSC and infrapatellar fat pad MSC | 6 months |
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Inclusion Criteria:
Patient who have an total knee replacement surgery
Exclusion Criteria:
Patient who don't sign the agreement
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Patient who have an total knee replacement surgery
| Name | Role | Phone | Extension | |
|---|---|---|---|---|
| Chih-Hung Chang, MD, Ph.D | Contact | 886-2-89667000 | 2888 | orthocch@mail.femh.org.tw |
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| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Far Eastern Memorial Hospital | Recruiting | Pan-Ciao | Taipei | 220 | Taiwan |
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| ID | Term |
|---|---|
| D007718 | Knee Injuries |
| ID | Term |
|---|---|
| D007869 | Leg Injuries |
| D014947 | Wounds and Injuries |
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