Effects of Ozone Therapy on Muscle Damage | NCT07728422

NCT07728422

Overview

NCT ID: NCT07728422
Lead Sponsor: Inonu UniversityOTHER
Official Title: Effects of Ozone Therapy Modalities on Biochemical Markers of Muscle Damage Following Eccentric Exercise
Brief Summary: The goal of this clinical trial is to determine the effectiveness of different ozone therapy methods in enhancing recovery after muscle-damaging exercise in healthy adults, and to evaluate their impact on biochemical markers of muscle damage and fatigue.

The main questions it aims to answer are:

Do ozone-based interventions accelerate recovery by reducing biochemical markers of muscle damage and fatigue following eccentric exercise?

Which ozone therapy modality provides the greatest recovery benefit? Researchers will compare three intervention groups (autohemotherapy, ozone sauna therapy, and ozonated lavender oil massage) with a control group receiving no treatment.

Participants will:

Complete a standardized eccentric exercise protocol designed to induce muscle damage

Receive one of the assigned interventions or no treatment (control)

Provide blood samples at multiple time points (baseline, post-exercise, post-intervention, and 24, 48, and 72 hours follow-up) to assess recovery- related biomarkers

Status and Timeline

Status: Completed
Last Update Posted: Jul 27, 2026Actual
Start Date: Oct 4, 2024Actual
Primary Completion Date: Nov 28, 2024Actual
Completion Date

Participation Criteria

Ages Eligible for Study: Adult (18-64)
Sexes Eligible for Study: Male
Accepts Healthy Volunteers?: Yes
Eligibility Criteria: Inclusion Criteria:

male football players aged 18-30 years

playing exclusively in the midfield position

active participation in regional professional football leagues (non-top-tier)

engagement in regular football training at least four sessions per week with a minimum duration of 90 minutes per session

absence of acute or chronic musculoskeletal injuries within the preceding six months

no use of anti-inflammatory medications or ergogenic supplements within the four weeks prior to the study

Exclusion Criteria:

female football players

diagnosed cardiovascular, metabolic, or neurological disorders

known hypersensitivity to ozone exposure or lavender oil

any surgical intervention within the past six months

concurrent participation in alternative recovery or therapeutic interventions during the study period

playing positions other than midfield

Study Plan

Study Type: Interventional
Phase: Not Applicable
Enrollment: 32Actual
Primary Purpose: Other
Allocation: Randomized
Interventional Model: Parallel Assignment
Masking: Single

Contacts and Locations

Central Contacts: No data available
Locations: Inonu University

Similar Studies

Participant Group/Arms

AutohemotherapyExperimental

Participants underwent major autohemotherapy involving withdrawal of venous blood, exposure to an oxygen-ozone mixture, and subsequent intravenous reinfusion in accordance with established clinical protocols

Other: Autohemotherapy

Ozone Sauna TherapyExperimental

Participants underwent ozone sauna therapy administered in a sealed cabin system, with the head outside the chamber to avoid inhalation, under controlled temperature and ozone concentration conditions.

Other: Ozone Sauna Therapy

Ozonated Lavender Oil MassageExperimental

Participants underwent massage therapy with ozonated lavender oil targeting lower extremity muscles, delivered by a certified therapist according to standardized procedures.

Other: Ozonated Lavender Oil Massage

ControlNo Intervention

Participants remained at rest without receiving any recovery intervention, serving as the control condition for comparison.