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Determine if acute supplementation with antioxidant vitamins (vitamin C and vitamin E) influences the muscle damage and fatigue caused after an acute session of eccentric training.
A randomized, single-blind, placebo-control parallel trial will be conducted. In a previous session volunteers will be interviewed to determine if they meet the inclusion criteria.
Participants should avoid ingesting of foods that contain high amounts of antioxidant compounds: more than two juices of fruits, more than four cups of coffee or tea. Juices with high antioxidant content should be avoided. Alcoholic beverages should also be avoided. Participants will be divided in to groups and will performance one resistance exercise sessions. Prior to exercise, one group will intake supplementation VitC (1000 mg) + VitE (235 mg) and the other supplementation with placebo.
The following sequence will be performed in each session: 1) baseline assessment (analysis of the composition body, blood draw and parameters of performance) prior to exercise session ; 2) administration of vitamin or placebo supplementation 2 hours before exercise session ; 3) post-exercise assessments (blood biomarkers and performance parameters) immediately after the session and after 24 hours. DOMS will also be assessed 48 h and 72 h after training. The training session implemented will be designed by a Sport Scientific
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Supplementation | Experimental | Vitamin C and Vitamin E before exercise |
|
| Placebo | Placebo Comparator | Placebo before exercise |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Dietary Supplement: Vitamin C and vitamin E | Dietary Supplement | Antioxidant supplementation |
|
| Measure | Description | Time Frame |
|---|---|---|
| Percentage of body fat and muscle mass | Determination of body composition using skinfolds measurements | Prior to the intervention |
| DOMS | Delayed onset muscle soreness. This outcome will be measure with a 10-point visual analogic scale (0= no pain; 10 = worst possible pain) | 0 hours post exercise |
| DOMS | Delayed onset muscle soreness. This outcome will be measure with a 10-point visual analogic scale (0= no pain; 10 = worst possible pain) | 24 hours post exercise |
| Blood malondialdehyde (MDA) | MDA will be analyzed from a blood sample | 2 hours prior to exercise |
| Blood creatine quinase (CK) | CK will be analyzed from a blood sample | 2 hours prior to exercise |
| Blood malondialdehyde (MDA) | MDA will be analyzed from a blood sample | Inmediatly after exercise |
| Blood malondialdehyde (MDA) | MDA will be analyzed from a blood sample | 24 hours after exercise |
| Blood creatine quinase (CK) | CK will be analyzed from a blood sample |
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Inclusion Criteria:
Exclusion Criteria:
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| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Universidad Europea de Madrid | Madrid | Spain |
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| ID | Term |
|---|---|
| D014810 | Vitamin E |
| D001205 | Ascorbic Acid |
| D024505 | Tocopherols |
| ID | Term |
|---|---|
| D001578 | Benzopyrans |
| D011714 | Pyrans |
| D006573 | Heterocyclic Compounds, 1-Ring |
| D006571 | Heterocyclic Compounds |
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| Placebo | Dietary Supplement | Placebo administration |
|
| 24 hours after exercise |
| Jump height in a Counter Movement Jump | Jump height (cm) in a counter movement jump measured with Octojump Platform | 2 hours prior to exercise |
| Jump height in a Counter Movement Jump | Jump height (cm) in a counter movement jump measured with Octojump Platform | Inmediatly after exercise |
| Jump height in a Counter Movement Jump | Jump height (cm) in a counter movement jump measured with Octojump Platform | 24 h after exercise |
| Jump Height in a Squat Jump | Jump Height (cm) in a Squat Jump measured with Octojump Platform | 24 h after exercise |
| Jump Height in a Squat Jump | Jump Height (cm) in a Squat Jump measured with Octojump Platform | Inmediately after exercise |
| Jump Height in a squat Jump | Jump height (cm) in a squat jump with Octojump Platform measured with Octojump Platform | 2 hours prior to exercise |
| Jump Height in a Drop Jump | Jump height (cm) was measured in different drop jumps with Octojump Platform | 2 hours prior to exercise |
| Jump Height in a Drop Jump | Jump height (cm) was measured in different drop jumps with Octojump Platform | Inmediately after exercise |
| Contact time in a Drop Jump | Contact time (s)measured in different drop jumps with Octojump Platform | Inmediately after exercise |
| Jump Height in a Drop Jump | Jump height (cm) measured in different drop jumps with Octojump Platform | Inmediately after exercise |
| Contact time in a Drop Jump | Contact time (s) measured in different drop jumps with Octojump Platform | 24 hours after exercise |
| Jump Height in a Drop Jump | Jump height (cm) measured in different drop jumps with Octojump Platform | 24 hours after exercise |
| Contact time in a Drop Jump | Capillary blood lactate evaluated with Lactate Pro 2 | Inmediately after exercise |
| Concentration of blood lactate | Capillary blood lactate with Lactate Pro 2 | 2 hours before exercise |
| D006574 |
| Heterocyclic Compounds, 2-Ring |
| D000072471 | Heterocyclic Compounds, Fused-Ring |
| D013400 | Sugar Acids |
| D000144 | Acids, Acyclic |
| D002264 | Carboxylic Acids |
| D009930 | Organic Chemicals |
| D006880 | Hydroxy Acids |
| D002241 | Carbohydrates |