Antimicrobial resistance genes (ARGs) are the genes that allow bacteria to survive antibiotic treatments. These genes can be found in beneficial gut microbes, and occur more frequently in infants than adults. These genes can also transmit between different bacterial species. Exclusively breastfed infants carry fewer ARGs than formula fed infants. We are trying to find out if care-givers working with exclusively breastfed infants have fewer ARGs than those working with formula fed infants.

Inclusion Criteria:

• Employment at a childcare facility or home daycare at time of sampling
• Participant works and interacts with infants under the age 6 months old, including responsibility for changing diapers
• Participant >18 years old

Exclusion Criteria:

• Participant has a history or presence of a medical condition including Ulcerative Colitis, Crohn's Disase, Inflammatory Bowel Disease, colon cancer, Celiac's disease, or other GI disordrs
• Current priobitic use or probiotic use within the last 4 weeks
• Participant is currently pregnant or breastfeeding
• Participant has an infant <12 months old at home for 3 or more days per week
• Enrollment in a clinical trial involving an invstigational drug or dvic
• Antibiotic use within the past 3 months
• Participant working exclusively with infants >6 months old at time of stool sample collection

Professional infant caregivers who work with infants at least 3 days a week for at least 6 hours per day who meet the inclusion and exclusion criteria of the sample.

We plan to post the host-subtracted metagenomic sequencing data in SRA or other biorepository. We will share the de-identified metadata used in analysis as supplements to any publications.