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The present project aims to elucidate the metabolic interactions within the tumor microenvironment of pancreatic adenocarcinoma, both in the primary tumor and in hepatic and pulmonary metastatic sites, as well as in circulating blood biomarkers.
Deciphering these complex interactions aims to identify exploitable vulnerabilities for predicting treatment efficacy or for guiding therapeutic strategies.
Pancreatic adenocarcinoma (PDAC) remains one of the deadliest cancers, with survival rarely exceeding a few months and limited progress despite improved chemotherapy. Its poor prognosis is driven by late diagnosis, therapeutic resistance, and low responsiveness to targeted therapies and immunotherapy.
PDAC is characterized by a dense, immunosuppressive stroma and severe metabolic stress due to poor vascularization. Tumor progression relies heavily on stromal cells-such as cancer-associated fibroblasts and macrophages-as well as metabolic adaptations of cancer cells.
This project focuses on two key PDAC features to identify new biomarkers and therapeutic targets:
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Groupe 1-Initial | Paired pancreatic and liver/lung metastatic biopsies will be used to establish primary and metastatic cell cultures from tumor specimens obtained during routine diagnostic procedures, along with peripheral blood samples, in a cohort of 12 patients |
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| Group 2- Relapse | Biopsies of liver or lung metastases obtained at metastatic relapse or progression, along with peripheral blood samples, will be required from 12 patients for the establishment of primary and metastatic cell cultures. |
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| Group 3- Biological | 40 patient samples collected before chemotherapy and paired samples collected during chemotherapy will support molecular and cellular analyses aimed at identifying and assessing novel biomarkers and therapeutic strategies |
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| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Biopsy collection | Other | Tumor cells isolation |
| |
| Measure | Description | Time Frame |
|---|---|---|
| Evaluation and comparison of biological parameters reflecting metabolic alterations within the tumor microenvironment of pancreatic adenocarcinoma |
These analyses aim to identify potential therapeutic targets and to explore diagnostic, prognostic, and predictive biomarkers of treatment response. | From sample collection to completion of analyses, estimated at 3 years |
| Measure | Description | Time Frame |
|---|---|---|
| Exploratory characterization of metabolic pathways, tumor microenvironment features, and associated biomarkers in pancreatic adenocarcinoma. |
|
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Inclusion Criteria:
Age > 18 years
Signed written informed consent specific to the PROMETAP study
Affiliation with a social security system
Treatment and follow-up performed at the Institut Paoli-Calmettes
Metastatic PDAC requiring biopsy as part of routine care (group1 and 2)
Prior treatment:
Eligible for chemotherapy (ECOG < 2)
Non-Inclusion Criteria:
5. Adult individuals under legal protection (guardianship, trusteeship, judicial protection) or unable to provide informed consent
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The study population will include adult men and women (≥18 years) diagnosed with metastatic pancreatic adenocarcinom
| Name | Role | Phone | Extension | |
|---|---|---|---|---|
| Jihane PAKRADOUNI | Contact | +334 91223778 | drci.up@ipc-unicancer.fr |
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| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Institut Paoli-Calmettes | Recruiting | Marseille | 13009 | France |
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| ID | Term |
|---|---|
| D001800 | Blood Specimen Collection |
| ID | Term |
|---|---|
| D013048 | Specimen Handling |
| D019411 | Clinical Laboratory Techniques |
| D019937 | Diagnostic Techniques and Procedures |
| D003933 | Diagnosis |
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Biopsy samples Blood samples
| Blood collection |
| Other |
Isolation of blood monocytes |
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| From sample collection to completion of analyses, estimated at 3 years |
| D011677 | Punctures |
| D013514 | Surgical Procedures, Operative |
| D008919 | Investigative Techniques |