Validation of SDS-EDTA-Treated Chromatography Paper Strips for Mpox Sampling and Transport in DRC

Use of SDS/EDTA-Treated Chromatography Strips for Sampling, Transportation and Laboratory Confirmation of Mpox Virus Infection

The objective of this prospective paired diagnostic comparison study is to evaluate the diagnostic yield and field applicability of SDS-EDTA-treated chromatography paper strips for the collection, transport, and laboratory detection of mpox virus compared with the routine swab-based sampling method under field conditions in the Democratic Republic of the Congo (DRC).

The study is conducted among patients with suspected mpox infection presenting to healthcare facilities in South Kivu, DRC. For each participant, paired samples are collected simultaneously using the standard swab method and the SDS-EDTA strip method. Samples are analyzed using locally available molecular diagnostic platforms.

Inclusion Criteria:

• Patients presenting with clinical signs compatible with mpox infection according to national case definition.
• Written informed consent obtained.
• Presence of at least two skin lesions in a similar stage of evolution suitable for paired sampling.

Exclusion Criteria:

• Refusal or inability to provide informed consent.
• Presence of only one suitable lesion for sampling.
• Lesions at markedly different stages of evolution.
• Inability to safely obtain paired samples.