This prospective longitudinal observational study aims to evaluate the diagnostic performance and agreement of Smartphone photography, intraoral 3D imaging, Quantitative Light-Induced Fluorescence Digital (QLF-D), and ICDAS clinical criteria for assessing non-cavitated caries lesion activity in post-orthodontic patients under a preventive follow-up program. Thirty participants aged 11 to 46 years with active initial caries lesions will be followed for three months. Clinical examinations, QLF-D imaging, smartphone photographs, and intraoral 3D scans will be obtained at baseline and follow-up visits combined with professional prophylaxis and oral hygiene reinforcement. The study will analyze changes in lesion activity, fluorescence parameters, and surface characteristics over time, as well as the agreement and diagnostic performance among the evaluated methods.

This prospective longitudinal observational study will evaluate the agreement and diagnostic performance of Smartphone photography, intraoral 3D imaging, Quantitative Light-Induced Fluorescence Digital (QLF-D), and ICDAS clinical criteria for assessing non-cavitated caries lesion activity in post-orthodontic patients.

Participants presenting active initial caries lesions on vestibular tooth surfaces will undergo clinical examinations, QLF-D imaging, smartphone photographs, and intraoral 3D scans at baseline and during a three-month preventive follow-up program. Professional prophylaxis and oral hygiene reinforcement will be performed throughout the study period.

Changes in clinical lesion activity, fluorescence parameters, and topographic characteristics will be analyzed to determine the monitoring capacity, agreement, and diagnostic performance of the evaluated methods. Patient acceptance of the diagnostic technologies will also be assessed.

Inclusion Criteria:

• Participants aged 11 to 46 years
• Completed fixed orthodontic treatment
• Presence of at least four active non-cavitated caries lesions (ICDAS 1-3)
• Lesions located on vestibular surfaces of anterior teeth and/or premolars
• Ability to attend follow-up visits during the three-month study period
• Signed informed consent and/or assent form

Exclusion Criteria:

• Systemic conditions affecting salivary flow or enamel structure
• Extensive cavitated lesions requiring restorative treatment
• Ongoing orthodontic treatment
• Use of medications that significantly affect oral conditions
• Inability to cooperate with imaging procedures or follow-up visits

The study population will include post-orthodontic patients from Bogotá, Colombia, aged 11 to 46 years, presenting active non-cavitated caries lesions on vestibular tooth surfaces. Participants will be recruited from university clinical settings and enrolled in a preventive follow-up program involving clinical and digital caries assessment methods.

Individual participant data will not be shared due to confidentiality and privacy considerations related to clinical images and personal health information collected during the study. De-identified aggregate results will be reported in scientific publications and presentations.