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| ID | Type | Description | Link |
|---|---|---|---|
| TDK-2023-13289 | Other Identifier | Atatürk University Scientific Research Projects |
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This study aims to investigate the role of the thioredoxin system in the development and progression of periodontitis. Researchers will examine the levels of specific antioxidant markers, such as thioredoxin reductase 1 (TrxR1), glutathione (GSH), and malondialdehyde (MDA), in gingival tissue samples collected from healthy individuals and patients with different stages of periodontal disease. The goal is to determine if TrxR1 levels can serve as a potential biomarker for diagnosing the severity and activity of periodontitis.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Healthy Control Group | Systemically and periodontally healthy individuals with no history of bone loss, probing depths ≤ 3 mm, and bleeding on probing < 10%. |
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| Stage 1-2 Periodontitis Group | Patients diagnosed with Stage 1 or Stage 2 periodontitis according to the 2017 World Workshop classification, characterized by interdental clinical attachment loss and radiographic bone loss. |
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| Stage 3-4 Periodontitis Group | Patients diagnosed with Stage 3 or Stage 4 periodontitis, characterized by severe clinical attachment loss, deep probing depths, and significant radiographic bone loss potentially leading to tooth loss. |
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| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Gingival Tissue Analysis | Other | Gingival tissue samples are collected from all participants. These samples are analyzed for Glutathione (GSH) and Malondialdehyde (MDA) levels using spectroscopic methods. Additionally, the expression of thioredoxin reductase 1 (TrxR1), thioredoxin (TXN), and thioredoxin-interacting protein (TXNIP) is determined through quantitative real-time PCR, western blot, and enzyme activity assays. |
| Measure | Description | Time Frame |
|---|---|---|
| Thioredoxin Reductase 1 (TrxR1) mRNA Expression Levels | The expression levels of TrxR1 in gingival tissue are determined by quantitative real-time PCR (qPCR). This measure evaluates the potential of TrxR1 as a biomarker for periodontitis severity. | Baseline (at the time of tissue collection) |
| Measure | Description | Time Frame |
|---|---|---|
| Thioredoxin (TXN) mRNA Expression Levels | Evaluation of TXN gene expression levels in gingival tissue samples using quantitative real-time PCR (qPCR) normalized to β-actin. | Baseline (at the time of tissue collection) |
| Thioredoxin-Interacting Protein (TXNIP) mRNA Expression Levels |
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Inclusion Criteria:
Exclusion Criteria:
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Patients visiting the Department of Periodontology at Atatürk University Faculty of Dentistry who meet the clinical criteria for health or periodontitis.
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| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Atatürk University Faculty of Dentistry | Erzurum | 25240 | Turkey (Türkiye) |
Individual participant data that underlie the results reported in this article, after de-identification, will be made available upon request.
Data will be available beginning 6 months and ending 36 months following article publication.
Proposals should be directed to turgdemir@hotmail.com. To gain access, data requestors will need to sign a data access agreement.
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| ID | Term |
|---|---|
| D010518 | Periodontitis |
| ID | Term |
|---|---|
| D010510 | Periodontal Diseases |
| D009059 | Mouth Diseases |
| D009057 | Stomatognathic Diseases |
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Evaluation of TXNIP gene expression levels in gingival tissue samples using quantitative real-time PCR (qPCR) normalized to β-actin. Time Frame: Baseline (at the time of tissue collection) |
| Baseline (at the time of tissue collection) |
| Malondialdehyde (MDA) Levels. | MDA levels in gingival tissue homogenates measured using the thiobarbituric acid reactive substances (TBARS) assay to evaluate lipid peroxidation. | Baseline (at the time of tissue collection). |
| Glutathione (GSH) Levels. | GSH levels in gingival tissue homogenates measured using spectroscopic methods to evaluate antioxidant status. | Baseline (at the time of tissue collection). |