This study will evaluate the effects of daily phosphatidylserine supplementation on electronic gaming performance, cognitive function, sleep quality, stress-related outcomes, and autonomic responses in healthy adult gamers. Participants will be enrolled in a randomized, double-blind, placebo-controlled, parallel-group study and assigned to receive placebo, 100 mg phosphatidylserine, or 200 mg phosphatidylserine daily for 6 weeks.

Outcome measures will be assessed at baseline, Week 3, and Week 6 and will include electronic gaming performance, cognitive function, visual analog scale ratings, sleep quality questionnaires, hemodynamic responses, and heart rate variability. The study is designed to determine whether daily phosphatidylserine supplementation can improve performance and selected physiological and psychological outcomes during prolonged electronic gaming sessions.

Electronic gaming has grown substantially in popularity, with increasing interest in nutritional strategies that may improve gaming performance, cognitive function, sleep quality, and stress management. Phosphatidylserine is a phospholipid commonly derived from soy that is highly concentrated in neural tissue and has been investigated for its potential effects on cognition, stress-related responses, and mental performance. Despite growing interest in nutritional support for gamers, limited controlled research has evaluated the effects of supplementation on gaming-specific performance and related physiological outcomes.

This study is a randomized, double-blind, placebo-controlled, parallel-group trial designed to examine the effects of phosphatidylserine supplementation on electronic gaming performance and associated cognitive, psychological, and physiological responses. Participants will be assigned to receive placebo, 100 mg phosphatidylserine, or 200 mg phosphatidylserine daily for 6 weeks. All participants and study personnel involved in testing and outcome assessment will remain blinded to treatment assignment throughout the study.

Following screening and informed consent, eligible participants will complete a familiarization session during which they will practice the cognitive tests and become oriented to the gaming environment and procedures. Participants will then complete testing visits at baseline, Week 3, and Week 6. Prior to each testing visit, participants will be instructed to replicate their dietary intake for the preceding 24 hours, maintain their normal caffeine intake pattern, refrain from other stimulants not commonly consumed in their diet for 48 hours, avoid caffeine doses greater than 100 mg within 3 hours of testing, and complete a 3-hour fast before arrival.

At each testing visit, body mass, resting heart rate, and resting blood pressure will be assessed. Participants will then complete sleep-related questionnaires, cognitive testing, and visual analog scales assessing fatigue, concentration, and focus. After ingestion of the assigned supplement dose, participants will undergo heart rate variability assessment and then complete two 60-minute electronic gaming sessions separated by interim testing. Hemodynamic responses, cognitive outcomes, and visual analog scale ratings will be reassessed after 60 minutes and again after 120 minutes of gaming. Heart rate variability will also be assessed following completion of the gaming session.

Electronic gaming performance will be assessed using a standardized video game protocol with predefined performance metrics recorded after each gaming period. Cognitive outcomes will include the Stroop Color and Word Test, Trail Making Test Parts A and B, and Profile of Mood States. Sleep-related outcomes will be assessed using validated questionnaires, and autonomic function will be evaluated using heart rate variability metrics. Hemodynamic responses will include heart rate and blood pressure measured under standardized conditions.

The primary objective of the study is to determine whether 6 weeks of phosphatidylserine supplementation improves electronic gaming performance, cognitive function, and subjective ratings related to fatigue, concentration, and focus compared with placebo. Secondary objectives include evaluating the effects of supplementation on sleep quality, heart rate variability, and hemodynamic responses.

Inclusion Criteria:

• 18 - 40 years of age
• Self-reported to be beginners to gaming or have no experience at all
• Body Mass Index between 18.5 - 29.9 kg/m2

Exclusion Criteria:

• Outside of age range
• Is considered a gamer
• Body mass index is outside of posted inclusion range
• Noncompliance with protocol
• Women who are currently pregnant or are actively planning to become pregnant

This study will employ a double-blind design in which participants, investigators, and study personnel involved in data collection and outcome assessment are blinded to group assignment. Study supplements will be provided in identical capsules and packaging to ensure blinding. Group assignments will be coded and maintained by an independent party not involved in study procedures or data analysis and will remain concealed until completion of data collection and primary analyses.