Nutritional clinical study to evaluate the absorption and presence of exogenous miRNAs from broccoli in plasma and peripheral blood mononuclear cells (PBMCs) and to determine their possible implication in the modulation of fecal miRNAs and gut microbiota in healthy volunteers.

Nutritional clinical study to evaluate the absorption and presence of exogenous miRNAs from broccoli in plasma and in peripheral blood mononuclear cells (PBMCs) and determine their possible involvement in the modulation of fecal miRNAs and gut microbiota in healthy volunteers. For this purpose, two nutritional interventions will be carried out: the first will consist of an acute intake to evaluate the pharmacokinetics of exogenous miRNAs of broccoli in human plasma, and the second will be a sustained daily intake of 200g broccoli for a duration of 2 weeks.

Inclusion Criteria:

• Men and women > 18 years and < 45 years
• Adequate cultural level and understanding of the clinical study.
• Agree to voluntarily participate in the study and give their written informed consent.

Exclusion Criteria:

• Intolerance to broccoli
• Having to follow some type of dietary restriction that prevents the consumption of broccoli.
• Not willing to consume the food in the required quantity and/or frequency.
• Subjects with severe disease (hepatic, renal, cardiovascular, etc.)
• Subjects under any type of pharmacological treatment.
• Having taken antibiotics 1 month before the start of the study (Specific for the 2-week long study).
• Pregnancy or breastfeeding.
• Subjects who refuse to collect stool samples or undergo the blood draws scheduled in the study.
• Not able or willing to travel to the study site.