Measurement of Pro-resolving Inflammation Biomarkers in Saliva, Gingival Fluid, and Dental Pulp in Patients With Symptomatic Pulpitis and Healthy Controls

Strategic Inflammation Modulation and Advanced Regenerative Therapy of Dental PULP

This observational study aims to better understand inflammation inside the dental pulp (the inner tissue of the tooth) in patients with symptomatic pulpitis compared with healthy individuals.

Researchers will measure inflammation-related biomarkers in saliva, gingival fluid, and dental pulp samples to determine whether these substances reflect the level of inflammation.

The study also evaluates whether saliva and oral fluids could be used as simple, non-invasive tools to detect and monitor pulp inflammation.

We hypothesize that patients with pulpitis will show different biomarker levels compared with healthy controls.

Inclusion criteria

• Age ≥ 18 years, male or female.
• Patient consulting for symptomatic pulpitis (confirmed clinical and radiographic diagnosis) or for wisdom tooth extraction (healthy subject).
• Patient able to understand the research and having signed a written consent form.
• Permanent teeth only.

Exclusion criteria

• Refusal to participate.
• Pregnancy or breastfeeding.
• Antibiotic or anti-inflammatory treatment in the week prior to sampling.
• Clinically diagnosed inflammatory gingival or periodontal disease (gingivitis or periodontitis)
• Chronic systemic inflammatory disease (e.g., uncontrolled diabetes, autoimmune diseases).
• Patients under guardianship, conservatorship, or legal protection