The current study primarily aims to evaluate the presence of T. parvum in periodontal health and disease, and its correlation with clinical parameters. Subgingival plaque samples will be collected using sterile paper points then the samples will be analyzed using quantitative polymerase chain reaction (qPCR) to detect T. parvum and estimate its relative amount in the biofilm. The results may help clarify the association between T. parvum and periodontitis.

Inclusion Criteria: include participants who have at least 20 teeth and diagnosed as generalized periodontitis.

Exclusion Criteria: participants meeting any of the following criteria were excluded: (1) undergoing orthodontic treatment; (2) having a history of antibiotic/nonsteroidal anti-inflammatory drugs use within the last three months; (3) having a history of valve replacement who need prophylactic antibiotic coverage; (4) pregnant or lactating women; (5) having a history periodontal therapy within the last six month.

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Study population involves those who are attending the clinics of Periodontics in the College of Dentistry / University of Baghdad, in Baghdad / Iraq, and seeking for periodontal treatment. Eligible patients according to the pre-defined inclusion and exclusion criteria who will be invited to participate in this study.