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A new classification of periodontal diseases was created in 2018. the investigators want to know if this clinical classification is based, or not, on a biological reality. To do this, the investigators will collect data from the clinical examination (clinical assessment and radiological assessment) as well as non-invasive samples of subgingival plaque and crevicular gingival fluid (CGF). The subgingival plaque samples will be analyzed to define the microbiotic profiles of the patients and the CGF determined to define their inflammatory expression profiles. These results will then be linked to the diagnosis of severity of periodontitis.
In the new classification of periodontal disease, the investigators will seek to know whether there is a specific bacterial signature associated with the severity of damage on the one hand, and the other hand, the investigators will search for cytokinic biomarkers which can be correlated with the severity of the damage.
In this study, the investigators propose to include 25 patients with periodontitis stage I and II versus stage III and IV according to Chicago classification (2018)
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| low severity | patient with periodontitis stage I and II |
| |
| high severity | patient with periodontitis stage III and IV |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| subgingival plaque sample | Other | for subgingival plaque sample, on three affected sites (PD>3mm) and three healthy sites (PD≤3mm), after supra-gingival plaque removal, sterile endodontic paper points were inserted in selects pockets for 30s to collect the subgingival plaque. The material was transferred into eppendorf tube, one for affected samples and one for healthy samples. |
| Measure | Description | Time Frame |
|---|---|---|
| Microbiota analysis of subgingival plaque | sample analysis by microbiome sequencing with 16S technics of subgingival plaque of affected sites and healthy sites | through study completion, an average of 1 year |
| Measure | Description | Time Frame |
|---|---|---|
| Cytokinic concentrations in CGF | cytokinic concentration analysis (IL1β, IL , TNFα, IL2, IL4, IL10, IL17α) in CGF of affected sites and healthy sites | through study completion, an average of 1 year |
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Inclusion Criteria:
Exclusion Criteria:
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All patient coming in consultation for generalized periodontitis .
| Name | Role | Phone | Extension | |
|---|---|---|---|---|
| Assem SOUEIDAN, Pr | Contact | 02.40.41.29.23 | +(33) | assem.soueidan@chu-nantes.fr |
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| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Nantes Universitary Hospital | Recruiting | Nantes | Loire Atlantique | 44093 | France |
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| ID | Term |
|---|---|
| D010518 | Periodontitis |
| D055113 | Chronic Periodontitis |
| D064806 | Dysbiosis |
| ID | Term |
|---|---|
| D010510 | Periodontal Diseases |
| D009059 | Mouth Diseases |
| D009057 | Stomatognathic Diseases |
| D002908 | Chronic Disease |
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subgingival plaque sample and gingival fluid sample
|
| CGF sample | Other | each site was sampled with a PerioPaper Strip® (Oraflow), and the volumes collected were measured with a Periotron® (Oraflow). Then samples of affected sites were stowed in an eppendorf tube with 120µL of PBS Tween. The same procedure was performed for the healthy sites |
|
| D020969 |
| Disease Attributes |
| D010335 | Pathologic Processes |
| D013568 | Pathological Conditions, Signs and Symptoms |