Periodontitis is a chronic inflammatory disease characterized by the progressive destruction of the supporting tissues of the teeth. Non-surgical periodontal therapy is the first-line treatment approach and aims to control inflammation and prevent further tissue breakdown.

The aim of this interventional clinical study is to evaluate the effects of non-surgical periodontal therapy on serum levels of tartrate-resistant acid phosphatase 5b (TRACP-5b), bone alkaline phosphatase (BALP), and 25-hydroxyvitamin D3 in patients diagnosed with Stage III periodontitis.

Patients with Stage III periodontitis will receive non-surgical periodontal therapy consisting of scaling and root planing. Clinical periodontal parameters and blood samples will be collected at baseline and after treatment. Changes in serum biomarker levels before and after therapy will be analyzed to assess the relationship between periodontal treatment and bone metabolism-related biomarkers.

The findings of this study are expected to contribute to a better understanding of the systemic biochemical response to periodontal therapy in patients with advanced periodontitis.

This interventional clinical study is designed to investigate the effects of non-surgical periodontal therapy on systemic bone metabolism-related biomarkers in patients diagnosed with Stage III periodontitis.

Eligible participants are systemically healthy individuals diagnosed with Stage III periodontitis according to the current periodontal classification. After enrollment, all participants will undergo non-surgical periodontal therapy consisting of scaling and root planing performed using standard clinical protocols.

Clinical periodontal parameters will be recorded at baseline and following the completion of periodontal therapy. Venous blood samples will be collected at baseline and at the post-treatment evaluation time point. Serum levels of tartrate-resistant acid phosphatase 5b (TRACP-5b), bone alkaline phosphatase (BALP), and 25-hydroxyvitamin D3 will be measured using standardized laboratory methods.

The primary objective of the study is to evaluate changes in serum levels of TRACP-5b, BALP, and 25-hydroxyvitamin D3 following non-surgical periodontal therapy. Secondary objectives include the assessment of clinical periodontal parameter changes and the exploration of associations between clinical findings and biochemical markers.

Data obtained before and after treatment will be analyzed to assess the systemic biochemical response to periodontal therapy. The results of this study may provide additional insight into the relationship between periodontal treatment and markers associated with bone metabolism.

Inclusion Criteria:

• Adults aged 18 to 65 years
• Diagnosed with Stage III periodontitis according to the current periodontal classification
• Systemically healthy individuals
• Presence of at least 20 natural teeth
• Willingness to participate in the study and provide written informed consent

Exclusion Criteria:

• Presence of systemic diseases that may affect periodontal status or bone metabolism
• Use of antibiotics, anti-inflammatory drugs, or medications affecting bone metabolism within the last 3 months
• Pregnancy or lactation
• Current smokers or history of smoking
• Previous periodontal treatment within the last 6 months

Individual participant data will not be shared due to patient confidentiality considerations and the single-center design of the study.