Exercise can reduce the risk of several chronic diseases. However, recent studies have suggested sitting for too long before exercising can decrease exercise benefits. This study will help determine why this may occur.

Previous work demonstrated a greater utilization of carbohydrates (CHO) at the end of aerobic exercise with a day of reduced activity (<5,000 steps). Additionally, there are differences in the RNA profile at rest and during exercise in pathways related to inflammation, metabolism, and innate immunity that warrant further research. Together, these data highlight that a single day of reduced activity alters systemic homeostasis that affects the exercise response. Given the potency of exercise to ameliorate risk of cardiovascular disease, more research is needed to find factors to augment the exercise response. Therefore, this study has two goals: 1) Analyze circulating metabolomics, and 2) metabolomics and proteomics in response to exercise after a day of reduced activity.

Inclusion Criteria:

• • between the ages of 18-30 years old

Exclusion Criteria:

• • free from acute or chronic illness (cardiac, pulmonary, liver, or kidney abnormalities, cancer, uncontrolled hypertension, insulin- or non-insulin dependent diabetes or other known metabolic disorders)

  • do not take medications related to metabolism (i.e., statins, metformin, GLP-1 agonists)
  • free from orthopedic limitations (including any artificial joints)
  • currently smoke or participate in other forms of tobacco use.