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| Name | Class |
|---|---|
| Charite University, Berlin, Germany | OTHER |
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The aim of the study is to evaluate circadian glucose, activity and dietary rhythms, as well as metabolic and cardiovascular risk associated with night shift work, sleep quality and well-being in a cohort of hospital staff, comparing employees with night shifts to employees without night shifts.
The parallel-arm study consists of group A of employees working night shifts and group B of employees not working night shifts. After a pre-screening and an inclusion examination, both groups attend their first visit after at least two day shifts before the start of their two-week observation phase. During the visit, anthropometric measurements are conducted, blood and hair samples are collected, and questionnaires are completed and handed out. During the observation period, the 24-hour activity and glucose rhythms of the study participants are measured and the food intake is documented. After an observation phase, group A attends a second visit after at least two night shifts, including blood and hair sample collection.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Group A | Night workers | ||
| Group B | Individuals not working night shifts |
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| Measure | Description | Time Frame |
|---|---|---|
| Mean 24-hour glucose levels and mean night glucose levels | Mean 24-hour glucose [mg/dl] and mean night glucose [mg/dl] assessed by continuous glucose monitoring (CGM) | 2 weeks |
| Measure | Description | Time Frame |
|---|---|---|
| Body weight | Body weight [kg], as measured by scale weight | 2 weeks |
| Body Mass Index (BMI) | BMI [kg/m2], calculated as weight/square height |
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Inclusion Criteria:
Exclusion Criteria:
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Hospital workers
| Name | Role | Phone | Extension | |
|---|---|---|---|---|
| Olga Ramich, Prof. Dr. | Contact | +49 33200 882749 | olga.ramich@dife.de | |
| Anna Merckens | Contact | +49 33200 882459 | anna.merckens@charite.de |
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| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Charité-Universitaetsmedizin Berlin, Department of Anesthesiology and Intensive Care Medicine (CCM/CVK) | Recruiting | Berlin | Germany |
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Blood, hair roots
| 2 weeks |
| Blood pressure | Systolic and diastolic blood pressure [mm Hg], as measured by manometer | 2 weeks |
| Glycemic variability | Inter- and intraday indices of glycemic variability assessed by CGM | 2 weeks |
| Eating time | Timing of all eating events [hh:mm] as assessed by food records | 2 weeks |
| Calorie intake | Calorie intake [kcal] as assessed by food records | 2 weeks |
| Food composition | Nutrient composition [EN%] as assessed by food records | 2 weeks |
| Subjective sleep quality | Sleep quality as assessed by the Pittsburgh Sleep Quality Index (PSQI score, which ranges from 0-21, where higher values correspond to worse sleep quality) | 2 weeks |
| Objective sleep quality | Sleep quality as assessed by the ActLumus device | 14 days |
| Sleep timing | Sleep timing (sleep onset and offset [hh:mm]) as monitored using a sleep diary | 14 days (sleep diary) |
| Body temperature | Body temperature is monitored continuously across the entire 24-hour cycle for two weeks by an ActLumus device | 2 weeks |
| Light exposure | Light exposure is monitored continuously across the entire 24-hour cycle for two weeks by an ActLumus device | 2 weeks |
| Physical activity | Physical activity is monitored continuously across the entire 24-hour cycle for two weeks by an ActLumus device | 2 weeks |
| Concentration of liver enzymes | γ-glutamyltransferase (GGT), aspartate aminotransferase (ALT) and alanine aminotransferase (AST) assessed in blood plasma | 2 weeks |
| Concentration of lipids | Total cholesterol, high-density lipoprotein and low-density lipoprotein cholesterol and triglycerides assessed in blood plasma | 2 weeks |
| Concentration of kidney parameters | Creatinine, urea and uric acid assessed in fasting blood samples | 2 weeks |
| Glucose concentration | Glucose levels [mg/dl] assessed in fasting blood samples | 2 weeks |
| HbA1C concentration | HbA1C [%] assessed in blood samples | 2 weeks |
| Insulin concentration | Levels of insulin assessed using ELISA in serum samples | 2 weeks |
| Cortisol concentration | Levels of cortisol assessed in blood serum | 2 weeks |
| Thyroid-stimulating hormone (TSH) concentration | Levels of TSH assessed in blood serum | 2 weeks |
| Leptin concentration | Levels of leptin assessed in blood serum | 2 weeks |
| Concentration of C-reactive protein (CRP) | CRP assessed in blood serum | 2 weeks |
| Concentration of interleukin-6 (IL-6) | IL-6 assessed in blood serum | 2 weeks |
| Gene expression in blood cells | Whole blood transcriptome assessed by RNAseq | 2 weeks |
| Internal circadian phase | Circadian phase as assessed using the HairTime assay in hair samples | 2 weeks |
| Chronotype | Chronotype will be determined using the Munich ChronoType Questionnaire (MCTQ) , with scores ranging from 16 to 86. Lower scores indicate extreme late chronotypes, while higher scores indicate extreme early chronotypes | 2 weeks |
| Pflegebereiche CC07 Charité - Universitätsmedizin Berlin Campus Virchow-Klinikum | Recruiting | Berlin | Germany |
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