Timing of Oocyte Retrieval and Micro Testicular Sperm Extraction

Timing of Oocyte Retrieval and Micro Testicular Sperm Extraction: Does it Make a Difference? A Single-center Retrospective Cohort

This is a retrospective study to assess outcomes of micro-TESE performed 24 or 48 hours before oocyte retrieval and ICSI.

This retrospective study evaluates patients undergoing ICSI following micro-TESE. Participants are assigned to two groups based on the interval between sperm extraction and oocyte retrieval (24 vs. 48 hours). Clinical and embryological outcomes from the first embryo transfer will be assessed.

Inclusion Criteria:

• Men who underwent a micro-TESE at the OVO clinic from June 2015 to January 2025.
• Patients with non-obstructive azoospermia (NOA), (absence of spermatozoa in multiple semen analyses)
• Patients with cryptozoospermia, (presence of a small number of spermatozoa detectable only after semen centrifugation)
• Patients with oligoasthenoteratozoospermia (OAT), (sperm concentration of less than 15 million/mL, reduced motility and abnormal morphology)

Exclusion Criteria:

• Patients who did not have any sperm noted following micro-TESE
• Use of donor sperm or oocytes
• Frozen sperm or oocytes
• Same day oocyte retrieval and sperm extraction
• Reversible cause of cryptozoospermia (i.e presence of a varicocele, febrile illness, or recent toxin exposure) Partial genital tract obstruction (i.e post-vaso-vasostomy, post-vasoepididymostomy, and low volume cryptozoospermia with ejaculatory duct obstruction)