fifteen patients with carious teeth showing signs and symptoms of pulp inflammation

fifteen patients with intact human premolars planed for extraction for orthodontic reason

1. Full medical and dental history will be taken from each patient.
2. Clinical and radiographic examination (Cone beam computed tomography) for the offending tooth.
3. After anesthesia, Rubber dam is applied and complete caries removal will be done, deeply inside the cavity, the excavation will be done using low speed hand piece and excavator.
4. A blood sample will be taken from the bleeding pulp tissue on a cotton pellet for Molecular test.
5. After exposure, the pulp will be amputated to the level of the canal orifices (full pulpotomy) pulp vitality was confirmed by the presence of bleeding pulp tissue from all canals.
6. Putty MTA will gently place over the pulp.
7. The cavity will be sealed with initial deep layer of resin modified glass ionomer restoration (RMGI) and final superficial layer of composite restoration.

post operative pain on cold testing binary outcome (Yes or no)

Cone Beam Computed Tomography CBCT radiographic Examination for any periodontal widening or any periapical radiolucency.