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The aim of this study is to investigate different resistance training loads on BNDF and irisin levels in platelets, blood serum and plasma.
In this study, the investigators will examine how three resistance training protocols acutely affect BDNF levels (in serum, plasma, platelet count, and BDNF per platelet) and irisin levels in blood (plasma). The resistance training protocols will differ in intensity, rest intervals between sets and repetition execution. Blood samples will be collected from participants at four time points: before the start of the protocol, 5 minutes after completion of the final set, 1 hour after completion of the final set, and 3 hours after completion of the final set. In addition, blood lactate levels will be monitored at the same time points. Heart rate will also be recorded (immediately before the start of the protocol and 30 seconds after completion of the final set). A control protocol will also be implemented, in which blood will be collected at the same time points. Data will be collected across five visits to the kinesiology laboratory at the University of Ljubljana, Faculty of Sport. Each visit will last approximately 4 hours, except for the first visit where 1RM will be measured.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Intervention: participants going through exercising or control | Experimental | All participants will do 1 control protocol and 3 resistance exercises protocols. Order of protocols will be randomized. |
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| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Exercise: 80% 1 RM with 2 minutes break | Other | Participant will do resistance exercise at 80% 1RM with 2 minutes break, 4 sets of each exercise. Included exercises are lat pull, bench press and leg press. |
| Measure | Description | Time Frame |
|---|---|---|
| plasma BDNF | BDNF will be determined in blood plasma with ELISA kit. | pre, 5 minutes post exercise, 1 hour post exercise, 3 hours post exercise. |
| Irisin | Irisin will be measured in blood plasma. | pre, 5 minutes post exercise, 1 hour post exercise, 3 hours post exercise. |
| serum BDNF | BDNF will be determined in blood serum with ELISA kit. | pre, 5 minutes post exercise, 1 hour post exercise, 3 hours post exercise. |
| Platelet count | Platelet count in blood will be done. | pre, 5 minutes post exercise, 1 hour post exercise, 3 hours post exercise. |
| BDNF per platelet | The amount of BDNF per platelet will be calculated. | pre, 5 minutes post exercise, 1 hour post exercise, 3 hours post exercise. |
| Measure | Description | Time Frame |
|---|---|---|
| Lactate | Lactate will be measured in blood. | pre, 5 minutes post exercise, 1 hour post exercise, 3 hours post exercise. |
| Stroop test | Stroop test will be used as an assessment of cognitive functions. The number of read word will be assessed. |
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Inclusion Criteria:
Exclusion Criteria:
Biological males.
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| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| University of Ljubljana, Faculty of Sport | Ljubljana | 1000 | Slovenia |
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| Exercise: 80% 1RM with1 minute break | Other | Participant will do resistance exercise at 80% 1RM with 1 minutes break, 4 sets of each exercise. Included exercises are lat pull, bench press and leg press. |
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| Exercise: accenuated eccentric exercise | Other | Participant will do accenuated eccentric resistance exercise at 80% 1RM in concetric part of movement and 110% 1RM in eccentric part of movement with 2 minutes break, 4 sets of each exercise. Included exercises are lat pull, bench press and leg press. |
|
| Control | Other | Timeline of events will be same as in exercise protocol (blood withdrawal and Stroop test), except participants will not do any kind of exercise protocol, instead they will be seating. |
|
| pre, 15 minutes post exercise. |
| ID | Term |
|---|---|
| D015444 | Exercise |
| D055070 | Resistance Training |
| ID | Term |
|---|---|
| D009043 | Motor Activity |
| D009068 | Movement |
| D009142 | Musculoskeletal Physiological Phenomena |
| D055687 | Musculoskeletal and Neural Physiological Phenomena |
| D005081 | Exercise Therapy |
| D012046 | Rehabilitation |
| D000359 | Aftercare |
| D003266 | Continuity of Patient Care |
| D005791 | Patient Care |
| D013812 | Therapeutics |
| D026741 | Physical Therapy Modalities |
| D064797 | Physical Conditioning, Human |
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