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| Name | Class |
|---|---|
| University Hospital of Split | OTHER |
| University of Split, School of Medicine | OTHER |
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This study will examine how hydration status (drinking a large amount of water or avoiding water for a short period of time) affects the eye. Healthy adult volunteers will participate in two sessions: one after mild dehydration (no water for 12 hours) and one after drinking 2 liters of water within 4 hours. At each session, detailed eye measurements will be taken, including scans of the retina and optic nerve using optical coherence tomography (OCT), corneal imaging with Pentacam, and eye length measurements with an optical biometer. The results from the two conditions will be compared within the same participants.
The main goal is to determine whether hydration influences the thickness of the retina (macula). Secondary goals include assessing changes in the optic nerve, cornea, and other biometric eye parameters. The study involves only non-invasive tests and minimal risk. Findings may help improve understanding of how hydration affects the eye and may be important for clinical practice, such as planning cataract or refractive surgery.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Relative Dehydration | Experimental | 12-hour fluid restriction (≤250 ml fluids until 20h previous day) |
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| Hyperhydration | Experimental | 2 liters of water within 4 hours before testing |
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| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Relative Dehydration Protocol | Behavioral | Participants will abstain from water intake for 12 hours prior to the study visit. Food is allowed, but fluid intake is limited to a maximum of 250 ml until 20:00 h on the previous evening. Eye measurements will be performed in the morning (8-10 h). |
| Measure | Description | Time Frame |
|---|---|---|
| Change in macular thickness between dehydration and hyperhydration states | Macular thickness (central subfield, parafoveal, and perifoveal regions) will be measured using Zeiss Cirrus HD-OCT. Each participant will undergo measurements in both hydration states, and paired comparisons will be performed. | Within 1 week (cross-over design with ≥7-day washout period) |
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Inclusion Criteria:
Exclusion Criteria:
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| Name | Role | Phone | Extension | |
|---|---|---|---|---|
| Ante Krešo, MD | Contact | +385 21 556 402 | ante.kreso@mefst.hr |
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| Hyperhydration Protocol | Behavioral | Participants will consume 2 liters of water within 4 hours prior to the study visit. Eye measurements will be performed in the morning (8-10 h). |
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