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| ID | Type | Description | Link |
|---|---|---|---|
| 308-2025 | Other Grant/Funding Number | Sakarya BAP |
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This study aims to compare the effects of aerobic and anaerobic exercise on hormonal, immunological, and metabolic biomarkers in young individuals using blood and saliva samples. It will also assess participants' physical activity levels, depression levels, and general lifestyle habits to explore their relationship with biomarker profiles. Biomarkers such as testosterone, progesterone, cortisol, IgA, alpha-amylase, insulin, lactate, and various inflammatory cytokines will be measured using ELISA. The study seeks to evaluate the physiological and psychosocial effects of different types of exercise in a holistic manner.
Exercise is a complex stimulus that induces multifaceted effects on an individual's physiological and psychological systems. Aerobic and anaerobic types of exercise differ in terms of their energy production pathways and metabolic responses in the body. Aerobic exercises are long-duration, oxygen-dependent activities, while anaerobic exercises are short-duration, high-intensity activities that activate oxygen-independent energy systems.
These types of exercise cause various hormonal, immunological, and metabolic changes in the body. After exercise, significant changes are observed in biomarkers such as stress hormones (cortisol, progesterone), anabolic hormones (testosterone), immune system markers (IgA, TNF-α, IL-6, IL-10, IL-1β, IL-1RA, IL-4), and metabolic parameters (amylase, insulin, lactate). The fact that these parameters can be measured from both blood and saliva samples has increased the use of non-invasive methods in exercise physiology studies.
The level of physical activity is directly related not only to biological systems but also to an individual's psychological health and lifestyle habits. Research shows that regular physical activity reduces levels of depression and improves quality of life. The positive effects of exercise on the immune system become more significant when considered in conjunction with an individual's general lifestyle habits (nutrition, sleep, stress levels, substance use, etc.). It is particularly known that individuals with depression have high cortisol levels and suppressed IgA levels. Therefore, depression levels and lifestyle habits are important variables that affect the physiological responses to exercise.
This study will compare the effects of aerobic and anaerobic exercise on hormonal, immunological, and metabolic biomarkers measured via blood and saliva samples in young individuals. Additionally, the participants' physical activity levels, depression levels, and general lifestyle habits will be evaluated to explore the relationship between these variables and biomarker profiles. Measurements will include levels of testosterone, progesterone, cortisol, IgA, alpha-amylase, insulin, lactate, and various inflammatory cytokines, assessed using the ELISA method. In this way, the biological effects of different types of exercise will be evaluated from both physiological and psychosocial perspectives.
The main aim of this study is to comparatively examine the effects of aerobic and anaerobic exercises on hormonal, immunological, and metabolic biomarkers measured through blood and saliva samples in young individuals. Additionally, the relationship between the participants' physical activity level, depression level, and general lifestyle habits (nutrition, sleep patterns, substance use, etc.) with these biomarkers will be investigated, as well as the potential modulatory effects on the physiological responses to exercise.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Group I - Control Group: | No Intervention | This group will not receive any training intervention. Participants will only undergo the Bruce Protocol and Wingate tests to serve as a baseline reference for physiological responses, allowing comparison with the other two groups. | |
| Group II - Treadmill Exercise (Bruce Protocol): | Experimental | Participants will perform the Bruce Protocol on a treadmill, which involves progressive stages of increasing speed and incline to challenge cardiovascular endurance. At each stage, heart rate, blood pressure, and fatigue level will be recorded. The session will end with a cool-down phase. |
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| Group III - Bicycle Exercise (Wingate Anaerobic Test): | Experimental | This group will perform the Wingate Anaerobic Test using a stationary exercise bike. Following a warm-up, participants will cycle at maximum effort for 30 seconds to assess anaerobic performance. Data collected will include pedaling speed, power output, heart rate, and oxygen saturation. |
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| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Treadmill Exercise | Device | Participants in this group will perform an aerobic exercise using the Bruce Protocol on a treadmill. The test involves walking or running with gradually increasing speed and incline in predefined stages to assess cardiovascular endurance. Heart rate, blood pressure, and fatigue levels will be recorded at each stage. The session will conclude with a low-intensity cool-down phase. |
| Measure | Description | Time Frame |
|---|---|---|
| Change in Salivary Testosterone Level | Measurement of testosterone concentration in saliva pre- and post-exercise. | Time Frame: Baseline and immediately post-exercise Unit of Measure: pg/mL |
| Change in Salivary Cortisol Level | Measurement of cortisol concentration in saliva before and after aerobic and anaerobic exercise protocols. | Time Frame: Baseline (pre-exercise) and immediately post-exercise (within 1 hour) Unit of Measure: ng/mL |
| Change in Salivary Progesterone Level | Salivary progesterone levels before and after exercise. | Time Frame: Baseline and immediately post-exercise Unit of Measure: pg/mL |
| Change in Salivary Alpha-Amylase Activity | Enzymatic activity of salivary alpha-amylase as a stress marker. | Time Frame: Baseline and post-exercise Unit of Measure: U/mL |
| Change in Blood Lactate Level | Lactate concentration in blood samples collected before and after exercise. | Time Frame: Baseline and post-exercise Unit of Measure: µIU/mL |
| Change in Serum Cytokine Levels (e.g., IL-6, TNF-α) | Evaluation of pro- and anti-inflammatory cytokines in serum. | Time Frame: Baseline and immediately post-exercise Unit of Measure: pg/mL |
| Change in Salivary Immunoglobulin A (IgA) | Level of IgA in saliva as a marker of mucosal immune response. |
| Measure | Description | Time Frame |
|---|---|---|
| Change in Depression Score (Beck Depression Inventory-II) | Assessment of participants' depressive symptoms using the validated Beck Depression Inventory-II (BDI-II). | Time Frame: Baseline (pre-intervention) and post-intervention (within 1 hour after final session) Unit of Measure: Score (range: 0-63) Method of Measurement: Self-reported questionnaire |
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Inclusion Criteria:
Exclusion Criteria:
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| Name | Role | Phone | Extension | |
|---|---|---|---|---|
| Abdurrahim Yıldız, Doc.Dr. | Contact | +90 507 751 63 63 | abdurrahimyildiz@subu.edu.tr |
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| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 33388079 | Result | Guest NS, VanDusseldorp TA, Nelson MT, Grgic J, Schoenfeld BJ, Jenkins NDM, Arent SM, Antonio J, Stout JR, Trexler ET, Smith-Ryan AE, Goldstein ER, Kalman DS, Campbell BI. International society of sports nutrition position stand: caffeine and exercise performance. J Int Soc Sports Nutr. 2021 Jan 2;18(1):1. doi: 10.1186/s12970-020-00383-4. | |
| 10893431 |
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| ID | Term |
|---|---|
| D009043 | Motor Activity |
| ID | Term |
|---|---|
| D001519 | Behavior |
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The study will be conducted in 2025 with 90 voluntary university students from the Department of Physiotherapy and Rehabilitation at Sakarya University of Applied Sciences. Participants will be aged 18-25 and free of chronic illnesses. Inclusion criteria are being a university student and within the specified age range. Exclusion criteria include orthopedic, cognitive, mental health problems, or chronic systemic diseases. Eligible students will be informed about the study's purpose, duration, potential risks, and methods, and will sign informed consent. Participants will be randomly divided into three equal groups, each receiving a different intervention, allowing comparison of physiological responses across groups.
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| Bicycle Exercise | Device | The Wingate Anaerobic Test will be conducted using an ergometer bike. After a standardized warm-up, participants will pedal all-out for 30 seconds against a preset resistance, aiming to evaluate their anaerobic performance. Physiological parameters including power output, heart rate, and oxygen saturation will be monitored throughout the test. |
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| Time Frame: Baseline and post-exercise Unit of Measure: µg/mL |
| Change in Physical Activity Level (IPAQ-Short Form) | Evaluation of participants' physical activity levels using the International Physical Activity Questionnaire - Short Form (IPAQ-SF), which estimates physical activity over the last 7 days in MET-minutes/week. | Time Frame: Baseline and post-intervention Unit of Measure: MET-minutes/week |
| Pedersen BK, Hoffman-Goetz L. Exercise and the immune system: regulation, integration, and adaptation. Physiol Rev. 2000 Jul;80(3):1055-81. doi: 10.1152/physrev.2000.80.3.1055. |
| 17303714 | Result | Gleeson M. Immune function in sport and exercise. J Appl Physiol (1985). 2007 Aug;103(2):693-9. doi: 10.1152/japplphysiol.00008.2007. Epub 2007 Feb 15. |
| 29150166 | Result | Mikkelsen K, Stojanovska L, Polenakovic M, Bosevski M, Apostolopoulos V. Exercise and mental health. Maturitas. 2017 Dec;106:48-56. doi: 10.1016/j.maturitas.2017.09.003. Epub 2017 Sep 7. |
| 29690792 | Result | Schuch FB, Vancampfort D, Firth J, Rosenbaum S, Ward PB, Silva ES, Hallgren M, Ponce De Leon A, Dunn AL, Deslandes AC, Fleck MP, Carvalho AF, Stubbs B. Physical Activity and Incident Depression: A Meta-Analysis of Prospective Cohort Studies. Am J Psychiatry. 2018 Jul 1;175(7):631-648. doi: 10.1176/appi.ajp.2018.17111194. Epub 2018 Apr 25. |