Subjects will brush their teeth in their usual manner for 1 timed minute twice daily with the toothbrush and toothpaste provided. They will apply the toothpaste on the full length of the toothbrush. After both brushing instances, subjects will rinse full strength with 20 mL of Mouthwash C for 30 timed seconds.

Dental Floss

Soft bristled, manual toothbrush

Sodium Monofluorophosphate 0.76 % (0.15% w/v fluoride ion) toothpaste

Dental plaque will be assessed at six sites for each graded tooth (mesiofacial, facial, distofacial, mesiolingual, lingual, distolingual) and scored as follows:

0=No plaque

1. Separate flecks of plaque at the cervical margin
2. A thin, continuous band of plaque (up to 1 mm) at the cervical margin
3. A band of plaque wider than 1 mm, but covering less than 1/3 of the side of the crown of the tooth
4. Plaque covering at least 1/3, but less than 2/3 of the side of the crown of the tooth
5. Plaque covering 2/3 or more of the side of the crown of the tooth

Interproximal mean includes mesiofacial, distofacial, mesiolingual, and distolingual sites. Higher scores indicate a worse outcome, per above.

Dental plaque will be assessed at six sites for each graded tooth (mesiofacial, facial, distofacial, mesiolingual, lingual, distolingual) and scored as follows:

0=No plaque

1. Separate flecks of plaque at the cervical margin
2. A thin, continuous band of plaque (up to 1 mm) at the cervical margin
3. A band of plaque wider than 1 mm, but covering less than 1/3 of the side of the crown of the tooth
4. Plaque covering at least 1/3, but less than 2/3 of the side of the crown of the tooth
5. Plaque covering 2/3 or more of the side of the crown of the tooth

Whole Mouth mean includes all six sites. Higher scores indicate a worse outcome, per above.

EBI will be assessed at six sites for each graded tooth (mesiofacial, facial, distofacial, mesiolingual, lingual, distolingual) by engaging a probe 1 mm into the gingival crevice and applying moderate pressure whilst sweeping from interproximal to interproximal along the sulcular epithelium. Interproximal mean includes mesiofacial, distofacial, mesiolingual, and distolingual sites. A higher score indicates a worse outcome, per below.

0 = no bleeding after 30 seconds

1. = bleeding upon probing after 30 seconds
2. = immediate bleeding observed

EBI will be assessed at six sites for each graded tooth (mesiofacial, facial, distofacial, mesiolingual, lingual, distolingual) by engaging a probe 1 mm into the gingival crevice and applying moderate pressure whilst sweeping from interproximal to interproximal along the sulcular epithelium. Whole Mouth mean includes all six sites. A higher score indicates a worse outcome, per below.

0 = no bleeding after 30 seconds

1. = bleeding upon probing after 30 seconds
2. = immediate bleeding observed

Bleeding on probing will be assessed at six sites for each graded tooth (mesiofacial, facial, distofacial, mesiolingual, lingual, distolingual) and scored as 1 = yes bleeding, 0 = no bleeding. Interproximal mean includes mesiofacial, distofacial, mesiolingual, and distolingual sites. A higher score indicates a worse outcome.

Bleeding on probing will be assessed at six sites for each graded tooth (mesiofacial, facial, distofacial, mesiolingual, lingual, distolingual) and scored as 1 = yes bleeding, 0 = no bleeding. Whole Mouth mean includes all six sites. A higher score indicates a worse outcome.

Probing depth will be measured at six sites for each graded tooth (mesiofacial, facial, distofacial, mesiolingual, lingual, distolingual) using a calibrated periodontal probe to ensure precision and consistency. Interproximal mean includes mesiofacial, distofacial, mesiolingual, and distolingual sites.

Measurements will be recorded to the nearest millimeter, with particular attention to maintaining gentle and consistent pressure to avoid measurement variability. Larger measurements indicate a worse condition.

Probing depth will be measured at six sites for each graded tooth (mesiofacial, facial, distofacial, mesiolingual, lingual, distolingual) using a calibrated periodontal probe to ensure precision and consistency. Interproximal mean includes mesiofacial, distofacial, mesiolingual, and distolingual sites.

Measurements will be recorded to the nearest millimeter, with particular attention to maintaining gentle and consistent pressure to avoid measurement variability. Larger measurements indicate a worse condition.