Evaluation of the Salivary-free Amino Acids Levels (Glycine , Proline , Lysine , and Alanine ) With Caries Activity

Evaluation of the Salivary-free Amino Acids Levels With Caries Activity

This study will be aimed to investigated the correlation between levels of salivary amino acids (glycine , proline , lysine , alanine ) in the saliva of healthy children with and without active carious lesions and to estimate the predictive capacity of caries susceptibility

80 healthy children (8-10 years old) will be recruited from primary schools of Baghdad city/ Iraq, will be classified according to their level of caries activity (high caries activity, n = 40; negative caries activity, n = 40). Total stimulated and unstimulated saliva will be collected to estimate the caries activity by using the Snyder test, and Amino acid analysis of the saliva will be performed by reversed phase - high performance liquid chromatography (RP-HPLC), respectively.

Inclusion Criteria:

• Children aged (8,9,10) years with different caries
• Absence of signs of gingivitis or periodontal disease.

Exclusion Criteria:

• Children out the range of age
• Children with any systemic discases.
• Children with appliances in the mouth.
• Children with appliances in the mouth.
• Children with antibiotic treatment or any other drug over the last two weeks. B