Development of Organoids From Primary Colorectal Tumors and Synchronous Liver Metastases

Pilot Feasibility Study for the Development of Organoids From Primary Colorectal Tumors and Synchronous Liver Metastases

Pilot feasibility study for the development of organoids from primary colorectal tumors and synchronous liver metastases, to prepare organoids from primary colorectal tumors and synchronous liver metastases. The organoids will allow us to study the function of genes targeted by the activity of the NF-Y/p53 complex.

Organoids will allow us to study the function of genes that are targets of the NF-Y/p53 complex activity. In these organoids, we will perform overexpression and/or silencing of these genes and assays of proliferation, invasion and motility. We will also perform functional assays in cocultures of the organoids with PBMCs from the same patients. This could allow the discovery of new pathways that could be targeted by targeted therapy and/or immunotherapy. Organoid preparation will be performed from fresh colorectal cancer tissue and liver metastasis following enzymatic digestion. Organoid culture medium and specific organic matrix will be used

Inclusion Criteria:

• Patients > 18 years
• Histological and cytological diagnosis of colorectal cancer neoplasia that has developed a liver metastasis
• Patients who are candidates for surgery for colorectal cancer with liver metastasis
• Signing of the relevant informed consent form.

Exclusion Criteria:

• All exclusion criteria adopted in surgical protocols will be applied to the study